Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4002522..4003141 Replicon chromosome
Accession NZ_CP031814
Organism Klebsiella pneumoniae strain KSB1_7F-sc-2280268

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag D0899_RS20260 Protein ID WP_002892050.1
Coordinates 4002923..4003141 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag D0899_RS20255 Protein ID WP_002892066.1
Coordinates 4002522..4002896 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0899_RS20245 3997674..3998867 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D0899_RS20250 3998890..4002036 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
D0899_RS20255 4002522..4002896 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
D0899_RS20260 4002923..4003141 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
D0899_RS20265 4003300..4003866 + 567 WP_002892030.1 maltose O-acetyltransferase -
D0899_RS28070 4003838..4003978 - 141 WP_004147370.1 hypothetical protein -
D0899_RS20270 4003999..4004469 + 471 WP_032431532.1 YlaC family protein -
D0899_RS20275 4004444..4005895 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
D0899_RS20280 4005996..4006694 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
D0899_RS20285 4006691..4006831 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
D0899_RS20290 4006831..4007094 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110215 WP_002892050.1 NZ_CP031814:4002923-4003141 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T110215 NZ_CP031814:4002923-4003141 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT110215 WP_002892066.1 NZ_CP031814:4002522-4002896 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT110215 NZ_CP031814:4002522-4002896 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAGGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References