Detailed information of TA system TA110205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1758776..1759366
• Replicon: chromosome
• Accession: NZ_CP031814
• Organism: Klebsiella pneumoniae strain KSB1_7F-sc-2280268

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: D0899_RS08715
• Protein ID: WP_023341911.1
• Coordinates: 1759034..1759366 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: D0899_RS08710
• Protein ID: WP_000288812.1
• Coordinates: 1758776..1759033 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0899_RS08675	1754708..1755364	-	657	WP_117387201.1	ATP-binding protein	-
D0899_RS08680	1755376..1756725	-	1350	WP_077254247.1	DNA (cytosine-5-)-methyltransferase	-
D0899_RS27545	1756733..1757149	-	417	WP_124047552.1	hypothetical protein	-
D0899_RS08690	1757479..1757877	+	399	WP_115657462.1	ash family protein	-
D0899_RS08695	1757777..1758238	+	462	WP_040210976.1	hypothetical protein	-
D0899_RS08700	1758235..1758441	+	207	WP_022615589.1	helix-turn-helix domain-containing protein	-
D0899_RS08710	1758776..1759033	+	258	WP_000288812.1	antitoxin	Antitoxin
D0899_RS08715	1759034..1759366	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D0899_RS08725	1759688..1761124	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
D0899_RS08735	1761490..1762944	-	1455	WP_004148975.1	AMP nucleosidase	-
D0899_RS08740	1763074..1763319	-	246	WP_023284006.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1753718..1754641	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T110205 WP_023341911.1 NZ_CP031814:1759034-1759366 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T110205 NZ_CP031814:1759034-1759366 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT110205 WP_000288812.1 NZ_CP031814:1758776-1759033 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT110205 NZ_CP031814:1758776-1759033 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0