Detailed information of TA system TA110201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 186724..187589
• Replicon: plasmid unnamed
• Accession: NZ_CP031809
• Organism: Klebsiella pneumoniae strain INF206-sc-2280074

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: D0896_RS27930
• Protein ID: WP_044350182.1
• Coordinates: 186724..187149 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: D0896_RS27935
• Protein ID: WP_117362324.1
• Coordinates: 187146..187589 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0896_RS27905	181824..183194	-	1371	WP_117362322.1	glutamine synthetase family protein	-
D0896_RS27910	183366..184217	+	852	WP_040217077.1	MurR/RpiR family transcriptional regulator	-
D0896_RS27915	184272..185021	+	750	WP_043875144.1	N-formylglutamate amidohydrolase	-
D0896_RS27920	185025..185672	+	648	WP_023328945.1	cysteine hydrolase	-
D0896_RS27925	185714..186682	+	969	WP_162889639.1	IS5 family transposase	-
D0896_RS27930	186724..187149	-	426	WP_044350182.1	RES family NAD+ phosphorylase	Toxin
D0896_RS27935	187146..187589	-	444	WP_117362324.1	DUF2384 domain-containing protein	Antitoxin
D0896_RS27940	187689..188411	-	723	Protein_183	DUF4113 domain-containing protein	-
D0896_RS27945	188578..189275	-	698	Protein_184	IS1 family transposase	-
D0896_RS27950	189442..189747	+	306	WP_023328947.1	chorismate mutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..233468	233468
	-	inside	IScluster/Tn	-	pla	185714..202741	17027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-132)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15587.47 Da
• Isoelectric Point: 4.0841

>T110201 WP_044350182.1 NZ_CP031809:c187149-186724 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVSDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSEFYGIVGFVE

Nucleotide

• Length: 426 bp

>T110201 NZ_CP031809:c187149-186724 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGTCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCGCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACTCTGAGTTTT
ACGGGATCGTGGGCTTTGTTGAATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16479.82 Da
• Isoelectric Point: 10.4564

>AT110201 WP_117362324.1 NZ_CP031809:c187589-187146 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYQVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT110201 NZ_CP031809:c187589-187146 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGATAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCCTATCAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA