Detailed information of TA system TA110196

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4724718..4725410
• Replicon: chromosome
• Accession: NZ_CP031808
• Organism: Klebsiella pneumoniae strain INF206-sc-2280074

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: D0896_RS23790
• Protein ID: WP_071603219.1
• Coordinates: 4724718..4725059 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: D0896_RS23795
• Protein ID: WP_040189932.1
• Coordinates: 4725084..4725410 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0896_RS23765	4720626..4722080	-	1455	WP_117362267.1	serine protease	-
D0896_RS23770	4722268..4723065	-	798	WP_038806919.1	helix-turn-helix transcriptional regulator	-
D0896_RS23775	4723166..4723441	-	276	Protein_4549	type II toxin-antitoxin system RelE/ParE family toxin	-
D0896_RS23780	4723445..4723693	-	249	WP_038806909.1	ribbon-helix-helix domain-containing protein	-
D0896_RS23785	4723770..4724603	-	834	WP_038806910.1	DUF4942 domain-containing protein	-
D0896_RS23790	4724718..4725059	-	342	WP_071603219.1	TA system toxin CbtA family protein	Toxin
D0896_RS23795	4725084..4725410	-	327	WP_040189932.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D0896_RS23800	4725424..4725900	-	477	WP_038806912.1	DNA repair protein RadC	-
D0896_RS23805	4725911..4726357	-	447	WP_038806913.1	antirestriction protein	-
D0896_RS23810	4726581..4727270	-	690	WP_038806914.1	hypothetical protein	-
D0896_RS23815	4727590..4727943	+	354	WP_038806915.1	hypothetical protein	-
D0896_RS23820	4727980..4728870	-	891	WP_117362268.1	50S ribosome-binding GTPase	-
D0896_RS23825	4728968..4730233	+	1266	WP_038806918.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4708195..4747600	39405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12762.63 Da
• Isoelectric Point: 7.8846

>T110196 WP_071603219.1 NZ_CP031808:c4725059-4724718 [Klebsiella pneumoniae]
MKTIPATTQRAAKSCPSPVAFWQMPLTRLLEQHYGLTLNDTPFSDETAIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQVTGLLRQSPHPSIR

Nucleotide

• Length: 342 bp

>T110196 NZ_CP031808:c4725059-4724718 [Klebsiella pneumoniae]
ATGAAAACCATACCTGCAACAACTCAGCGGGCGGCGAAGTCGTGCCCGTCGCCCGTGGCTTTCTGGCAGATGCCACTTAC
CCGTCTGCTGGAGCAGCATTATGGCCTCACCCTGAATGACACGCCGTTCAGCGATGAAACCGCTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AACTGTCAGGAGCAGTCCCCTTATCTGGGTGCAGTGGATATTTTGCGGGCGCGGCAGGTAACCGGCCTGTTGCGGCAAAG
CCCTCATCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12333.06 Da
• Isoelectric Point: 6.9560

>AT110196 WP_040189932.1 NZ_CP031808:c4725410-4725084 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFVDAECQKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT110196 NZ_CP031808:c4725410-4725084 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGTTGATGCCGAATGTCAGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGCAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA