Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4058422..4059041 Replicon chromosome
Accession NZ_CP031808
Organism Klebsiella pneumoniae strain INF206-sc-2280074

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag D0896_RS20490 Protein ID WP_002892050.1
Coordinates 4058823..4059041 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag D0896_RS20485 Protein ID WP_002892066.1
Coordinates 4058422..4058796 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0896_RS20475 4053574..4054767 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D0896_RS20480 4054790..4057936 + 3147 WP_004147373.1 multidrug efflux RND transporter permease subunit -
D0896_RS20485 4058422..4058796 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
D0896_RS20490 4058823..4059041 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
D0896_RS20495 4059200..4059766 + 567 WP_002892030.1 maltose O-acetyltransferase -
D0896_RS29020 4059738..4059878 - 141 WP_004147370.1 hypothetical protein -
D0896_RS20500 4059899..4060369 + 471 WP_002892026.1 YlaC family protein -
D0896_RS20505 4060344..4061795 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
D0896_RS20510 4061896..4062594 + 699 WP_040220001.1 GNAT family N-acetyltransferase -
D0896_RS20515 4062591..4062731 - 141 WP_040219999.1 type B 50S ribosomal protein L36 -
D0896_RS20520 4062731..4062994 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110194 WP_002892050.1 NZ_CP031808:4058823-4059041 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T110194 NZ_CP031808:4058823-4059041 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT110194 WP_002892066.1 NZ_CP031808:4058422-4058796 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT110194 NZ_CP031808:4058422-4058796 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References