Detailed information of TA system TA110119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 23030..23766
• Replicon: plasmid unnamed1
• Accession: NZ_CP031793
• Organism: Klebsiella pneumoniae strain INF116-sc-2279924

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D0883_RS26330
• Protein ID: WP_016240380.1
• Coordinates: 23284..23766 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: D0883_RS26325
• Protein ID: WP_003026799.1
• Coordinates: 23030..23296 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0883_RS26300	18618..19832	-	1215	WP_117093863.1	D-galactonate dehydratase family protein	-
D0883_RS26305	20302..20556	+	255	WP_117093862.1	hypothetical protein	-
D0883_RS26310	20553..21773	+	1221	WP_117093861.1	hypothetical protein	-
D0883_RS26315	21892..22386	+	495	WP_117093860.1	DNA-binding protein	-
D0883_RS26325	23030..23296	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
D0883_RS26330	23284..23766	+	483	WP_016240380.1	GNAT family N-acetyltransferase	Toxin
D0883_RS26335	23978..25288	+	1311	WP_117380947.1	ISNCY family transposase	-
D0883_RS26340	25483..26187	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
D0883_RS26345	26464..27780	+	1317	Protein_22	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..141675	141675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17323.95 Da
• Isoelectric Point: 9.5822

>T110119 WP_016240380.1 NZ_CP031793:23284-23766 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T110119 NZ_CP031793:23284-23766 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT110119 WP_003026799.1 NZ_CP031793:23030-23296 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT110119 NZ_CP031793:23030-23296 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCCCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66