Detailed information of TA system TA110104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1761703..1762293
• Replicon: chromosome
• Accession: NZ_CP031792
• Organism: Klebsiella pneumoniae strain INF116-sc-2279924

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: D0883_RS08730
• Protein ID: WP_023341911.1
• Coordinates: 1761961..1762293 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: D0883_RS08725
• Protein ID: WP_000288812.1
• Coordinates: 1761703..1761960 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0883_RS28490	1757529..1757852	-	324	WP_016532370.1	hypothetical protein	-
D0883_RS08700	1758038..1759786	+	1749	WP_117058118.1	hypothetical protein	-
D0883_RS08705	1759865..1760422	+	558	WP_020805022.1	hypothetical protein	-
D0883_RS08715	1761162..1761368	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
D0883_RS08725	1761703..1761960	+	258	WP_000288812.1	antitoxin	Antitoxin
D0883_RS08730	1761961..1762293	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D0883_RS08740	1762615..1764051	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
D0883_RS08750	1764417..1765871	-	1455	WP_004148975.1	AMP nucleosidase	-
D0883_RS08755	1766001..1766246	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T110104 WP_023341911.1 NZ_CP031792:1761961-1762293 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T110104 NZ_CP031792:1761961-1762293 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT110104 WP_000288812.1 NZ_CP031792:1761703-1761960 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT110104 NZ_CP031792:1761703-1761960 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0