Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 189040..189950 Replicon plasmid unnamed1
Accession NZ_CP031790
Organism Klebsiella pneumoniae strain KSB1_1I-sc-2280289

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag D0876_RS26915 Protein ID WP_004144067.1
Coordinates 189040..189510 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag D0876_RS26920 Protein ID WP_004026357.1
Coordinates 189507..189950 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0876_RS26885 184066..184443 + 378 WP_032719541.1 hypothetical protein -
D0876_RS28870 184640..185614 - 975 WP_048291873.1 hypothetical protein -
D0876_RS26895 186219..186380 - 162 WP_004118124.1 type I toxin-antitoxin system Hok family toxin -
D0876_RS26900 187787..187945 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
D0876_RS26910 188669..188929 + 261 WP_048291871.1 hypothetical protein -
D0876_RS26915 189040..189510 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
D0876_RS26920 189507..189950 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
D0876_RS26925 190051..190557 - 507 Protein_187 DUF4113 domain-containing protein -
D0876_RS26930 190658..190879 - 222 WP_072060588.1 DUF2188 domain-containing protein -
D0876_RS26935 191303..192040 + 738 Protein_189 DUF1311 domain-containing protein -
D0876_RS26940 192040..192381 + 342 WP_060598810.1 hypothetical protein -
D0876_RS26950 192776..193821 + 1046 Protein_191 IS3 family transposase -
D0876_RS28875 193876..194250 + 375 WP_181940019.1 hypothetical protein -
D0876_RS28880 194180..194404 + 225 WP_127807699.1 DUF4113 domain-containing protein -
D0876_RS26960 194483..194711 + 229 Protein_194 type II toxin-antitoxin system Phd/YefM family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS 1..213061 213061
- flank IS/Tn - - 193123..193821 698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T110099 WP_004144067.1 NZ_CP031790:c189510-189040 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T110099 NZ_CP031790:c189510-189040 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTAATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT110099 WP_004026357.1 NZ_CP031790:c189950-189507 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT110099 NZ_CP031790:c189950-189507 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTCCTGGGGCAAATCAATGAAGGCCTGGACGGGAAGGTGGCAAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCTTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAATGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACACGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References