Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 144872..145676 Replicon plasmid unnamed1
Accession NZ_CP031790
Organism Klebsiella pneumoniae strain KSB1_1I-sc-2280289

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag D0876_RS26665 Protein ID WP_072198182.1
Coordinates 145149..145676 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag D0876_RS26660 Protein ID WP_048264996.1
Coordinates 144872..145141 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0876_RS26645 140125..141117 + 993 WP_117394451.1 ABC transporter substrate-binding protein -
D0876_RS26650 141114..141860 + 747 WP_048978068.1 ABC transporter permease -
D0876_RS26655 141875..144238 + 2364 WP_060598671.1 hypothetical protein -
D0876_RS26660 144872..145141 + 270 WP_048264996.1 DUF1778 domain-containing protein Antitoxin
D0876_RS26665 145149..145676 + 528 WP_072198182.1 GNAT family N-acetyltransferase Toxin
D0876_RS28700 145760..146068 + 309 WP_048291890.1 hypothetical protein -
D0876_RS26675 146119..146816 + 698 Protein_141 IS1 family transposase -
D0876_RS26680 147128..148030 - 903 WP_048265000.1 LysR family transcriptional regulator -
D0876_RS26685 148128..148736 + 609 WP_049095464.1 short chain dehydrogenase -
D0876_RS26690 148855..150036 - 1182 WP_060598593.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS 1..213061 213061
- flank IS/Tn - - 146313..146816 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(54-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 20078.04 Da        Isoelectric Point: 5.7389

>T110097 WP_072198182.1 NZ_CP031790:145149-145676 [Klebsiella pneumoniae]
VMQELAIEMINDEFDYDIGEFDCGEETLNTFLKDHLKRQHDGQILKGYVLVTKEQKPKLLGYYTLSGSCFERKMLPSKTQ
QRKIPYQNAPSITLGRLAIDKSIQGQGWGELLVTHAMNVVWDASKAVGIYGLFVEALNDKAKRFYTRLDFIQLVGENSNL
LFYPTKSIEKIFTEE

Download         Length: 528 bp

>T110097 NZ_CP031790:145149-145676 [Klebsiella pneumoniae]
GTGATGCAAGAGCTGGCGATAGAGATGATCAACGACGAATTTGACTATGACATAGGCGAATTCGATTGTGGAGAAGAAAC
CTTAAATACATTTTTGAAAGACCATCTCAAGCGGCAGCATGATGGGCAAATTTTAAAAGGTTATGTATTAGTAACCAAAG
AACAGAAGCCTAAGCTGTTAGGCTATTACACATTATCCGGGAGCTGTTTCGAGAGAAAAATGCTCCCGTCAAAAACACAG
CAAAGAAAAATCCCTTATCAGAATGCGCCAAGCATTACTTTGGGACGCTTGGCTATTGACAAATCAATTCAAGGTCAGGG
ATGGGGTGAATTGCTAGTAACTCACGCAATGAATGTCGTCTGGGATGCATCTAAGGCCGTAGGAATATATGGTCTCTTCG
TGGAAGCTCTGAACGATAAAGCTAAGCGCTTCTATACCAGATTAGATTTCATACAGCTCGTTGGTGAGAATAGTAATCTG
CTATTTTACCCGACAAAATCTATTGAAAAAATCTTTACCGAAGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10148.65 Da        Isoelectric Point: 10.1733

>AT110097 WP_048264996.1 NZ_CP031790:144872-145141 [Klebsiella pneumoniae]
MSALRKQRIDLRLTDDDKSLIEEAAAMSNQTITQFVVASASERAAEVIEQHRRMVLNEESWNLVMDAIANPAAPNERLKR
AAKRLQKMR

Download         Length: 270 bp

>AT110097 NZ_CP031790:144872-145141 [Klebsiella pneumoniae]
ATGTCCGCACTGAGAAAGCAGCGCATAGATCTAAGATTAACTGATGACGACAAAAGCTTAATCGAAGAAGCCGCGGCAAT
GTCAAACCAGACTATTACACAGTTTGTAGTTGCCAGTGCCTCAGAACGTGCAGCGGAAGTGATTGAACAACATCGCAGAA
TGGTTCTAAACGAGGAGTCTTGGAACCTTGTCATGGATGCGATCGCAAACCCTGCTGCGCCGAATGAGCGGCTGAAGCGA
GCAGCGAAGCGTCTACAAAAAATGAGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References