Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2659042..2659693 Replicon chromosome
Accession NZ_CP031785
Organism Lacticaseibacillus paracasei strain 10266

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag D0638_RS13645 Protein ID WP_003567661.1
Coordinates 2659042..2659425 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag D0638_RS13650 Protein ID WP_003567665.1
Coordinates 2659445..2659693 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0638_RS13640 2658337..2658858 - 522 WP_003567657.1 QueT transporter family protein -
D0638_RS13645 2659042..2659425 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D0638_RS13650 2659445..2659693 - 249 WP_003567665.1 antitoxin Antitoxin
D0638_RS13655 2659761..2660897 - 1137 WP_003581082.1 alanine racemase -
D0638_RS13660 2660884..2661258 - 375 WP_003567669.1 holo-ACP synthase -
D0638_RS13665 2661427..2662935 - 1509 WP_149350294.1 DEAD/DEAH box helicase -
D0638_RS15780 2663061..2663204 - 144 WP_172623759.1 hypothetical protein -
D0638_RS13670 2663235..2664623 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T110076 WP_003567661.1 NZ_CP031785:c2659425-2659042 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T110076 NZ_CP031785:c2659425-2659042 [Lacticaseibacillus paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCAGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGCTTGGTTAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT110076 WP_003567665.1 NZ_CP031785:c2659693-2659445 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT110076 NZ_CP031785:c2659693-2659445 [Lacticaseibacillus paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCGGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References