Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3761120..3761756 Replicon chromosome
Accession NZ_CP031784
Organism Bacillus subtilis strain HMNig-2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D0819_RS19485 Protein ID WP_003156187.1
Coordinates 3761406..3761756 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag D0819_RS19480 Protein ID WP_003225183.1
Coordinates 3761120..3761401 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0819_RS19460 3757479..3758078 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
D0819_RS19465 3758173..3758538 + 366 WP_015252768.1 holo-ACP synthase -
D0819_RS19470 3758704..3759720 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
D0819_RS19475 3759835..3761004 + 1170 WP_015252766.1 alanine racemase -
D0819_RS19480 3761120..3761401 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D0819_RS19485 3761406..3761756 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D0819_RS19490 3761872..3762696 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
D0819_RS19495 3762701..3763066 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
D0819_RS19500 3763070..3763471 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
D0819_RS19505 3763483..3764490 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
D0819_RS19510 3764559..3764888 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
D0819_RS19515 3764885..3765367 + 483 WP_021481702.1 anti-sigma B factor RsbW -
D0819_RS19520 3765333..3766121 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
D0819_RS19525 3766121..3766720 + 600 WP_021481701.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T110074 WP_003156187.1 NZ_CP031784:3761406-3761756 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T110074 NZ_CP031784:3761406-3761756 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT110074 WP_003225183.1 NZ_CP031784:3761120-3761401 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT110074 NZ_CP031784:3761120-3761401 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References