Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1836705..1837621 Replicon chromosome
Accession NZ_CP031783
Organism Bacillus subtilis strain MENO2

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag D0808_RS09555 Protein ID WP_003244695.1
Coordinates 1836875..1837621 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag D0808_RS09550 Protein ID WP_003232646.1
Coordinates 1836705..1836875 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0808_RS09515 1833569..1833898 + 330 WP_153255952.1 XkdW family protein -
D0808_RS09520 1833895..1834059 + 165 WP_041850927.1 XkdX family protein -
D0808_RS09525 1834103..1834942 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
D0808_RS09530 1834995..1835264 + 270 WP_003232655.1 hemolysin XhlA family protein -
D0808_RS09535 1835277..1835540 + 264 WP_003232653.1 phage holin -
D0808_RS09540 1835553..1836446 + 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
D0808_RS09545 1836483..1836620 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
D0808_RS09550 1836705..1836875 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
D0808_RS09555 1836875..1837621 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
D0808_RS09560 1837731..1838732 - 1002 WP_003232642.1 inorganic phosphate transporter -
D0808_RS09565 1838745..1839362 - 618 WP_003218470.1 DUF47 domain-containing protein -
D0808_RS09570 1839638..1840954 - 1317 WP_085186195.1 serine/threonine exchanger -
D0808_RS09575 1841342..1842292 + 951 WP_041344800.1 ring-cleaving dioxygenase -
D0808_RS09580 1842393..1842539 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1802790..1858104 55314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T110062 WP_003244695.1 NZ_CP031783:c1837621-1836875 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T110062 NZ_CP031783:c1837621-1836875 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGTGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT110062 WP_003232646.1 NZ_CP031783:c1836875-1836705 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT110062 NZ_CP031783:c1836875-1836705 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References