Detailed information of TA system TA110061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1045901..1046537
• Replicon: chromosome
• Accession: NZ_CP031783
• Organism: Bacillus subtilis strain MENO2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: D0808_RS05320
• Protein ID: WP_003156187.1
• Coordinates: 1046187..1046537 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: D0808_RS05315
• Protein ID: WP_003225183.1
• Coordinates: 1045901..1046182 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0808_RS05295	1042260..1042859	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
D0808_RS05300	1042954..1043319	+	366	WP_015252768.1	holo-ACP synthase	-
D0808_RS05305	1043485..1044501	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
D0808_RS05310	1044616..1045785	+	1170	WP_017697002.1	alanine racemase	-
D0808_RS05315	1045901..1046182	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
D0808_RS05320	1046187..1046537	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D0808_RS05325	1046652..1047476	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
D0808_RS05330	1047481..1047846	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
D0808_RS05335	1047850..1048251	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
D0808_RS05340	1048263..1049270	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
D0808_RS05345	1049332..1049661	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
D0808_RS05350	1049658..1050140	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
D0808_RS05355	1050106..1050894	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
D0808_RS05360	1050894..1051493	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T110061 WP_003156187.1 NZ_CP031783:1046187-1046537 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T110061 NZ_CP031783:1046187-1046537 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT110061 WP_003225183.1 NZ_CP031783:1045901-1046182 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT110061 NZ_CP031783:1045901-1046182 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32