Detailed information of TA system TA110056

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4576849..4577655
• Replicon: chromosome
• Accession: NZ_CP031780
• Organism: Yersinia pseudotuberculosis strain FDAARGOS_342

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CEQ20_RS21335
• Protein ID: WP_057532451.1
• Coordinates: 4577272..4577655 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3G5KEF9
• Locus tag: CEQ20_RS21330
• Protein ID: WP_050090622.1
• Coordinates: 4576849..4577217 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ20_RS21290	4571878..4572096	-	219	WP_057532456.1	helix-turn-helix domain-containing protein	-
CEQ20_RS21295	4572191..4572838	-	648	WP_057532447.1	inovirus Gp2 family protein	-
CEQ20_RS21300	4573437..4574414	+	978	WP_123784591.1	hypothetical protein	-
CEQ20_RS21305	4574490..4575323	+	834	WP_057532449.1	DUF945 domain-containing protein	-
CEQ20_RS21310	4575335..4575558	+	224	Protein_3958	DUF905 domain-containing protein	-
CEQ20_RS21315	4575633..4576103	+	471	WP_057532450.1	antirestriction protein	-
CEQ20_RS21320	4576113..4576586	+	474	WP_050090624.1	DNA repair protein RadC	-
CEQ20_RS21325	4576596..4576817	+	222	WP_050090623.1	DUF987 domain-containing protein	-
CEQ20_RS21330	4576849..4577217	+	369	WP_050090622.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CEQ20_RS21335	4577272..4577655	+	384	WP_057532451.1	TA system toxin CbtA family protein	Toxin
CEQ20_RS21340	4577652..4578143	+	492	WP_050090620.1	hypothetical protein	-
CEQ20_RS23130	4578157..4578318	-	162	WP_155407244.1	hypothetical protein	-
CEQ20_RS21345	4578317..4579165	+	849	WP_057532452.1	DUF4942 domain-containing protein	-
CEQ20_RS21350	4579261..4579509	+	249	WP_050090618.1	ribbon-helix-helix domain-containing protein	-
CEQ20_RS21355	4579513..4579809	+	297	WP_072080376.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CEQ20_RS21360	4580147..4581382	+	1236	WP_072091827.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4563816..4579809	15993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-116)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14375.49 Da
• Isoelectric Point: 8.9747

>T110056 WP_057532451.1 NZ_CP031780:4577272-4577655 [Yersinia pseudotuberculosis]
MQTLPLTPQRAASSCPSPVEIWQKLLTHLLEQHYGLTLSDTPFSNETTIREHIDAGISLSDAVNFLVEKYELVRTDQPGF
TLMKQSPFITPIDILRARQATGLMKRRGYKAVSDITRGKASRMEQHH

Nucleotide

• Length: 384 bp

>T110056 NZ_CP031780:4577272-4577655 [Yersinia pseudotuberculosis]
ATGCAAACTTTACCTTTAACCCCGCAACGGGCGGCTTCGTCCTGCCCGTCACCTGTTGAAATATGGCAAAAACTGCTGAC
ACATCTGCTCGAACAACACTATGGTCTGACGCTCAGTGATACTCCATTCAGCAATGAAACCACCATTCGCGAACATATCG
ACGCGGGGATCTCCCTCAGCGATGCGGTGAACTTTCTGGTGGAGAAGTATGAACTGGTGCGCACTGACCAGCCTGGTTTC
ACCTTAATGAAGCAGTCACCGTTTATCACTCCAATCGATATTCTCCGCGCCAGGCAAGCCACCGGTCTGATGAAGCGCAG
GGGTTATAAAGCGGTGAGTGATATTACCCGTGGCAAAGCGAGTCGGATGGAGCAACACCATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13494.34 Da
• Isoelectric Point: 5.5999

>AT110056 WP_050090622.1 NZ_CP031780:4576849-4577217 [Yersinia pseudotuberculosis]
MKSVALSGIPPEASPCQQWGLNREVTPVFGARLVQEGNRLHFLTDRAGFNGAFSEDDALRLDQAFPLMLKQLEIELRSGE
LNPRRQHCVTLNHNGLTCEADTLGSYGYVYIAIYPTSLCSPV

Nucleotide

• Length: 369 bp

>AT110056 NZ_CP031780:4576849-4577217 [Yersinia pseudotuberculosis]
ATGAAATCTGTCGCCCTGTCCGGTATACCACCGGAGGCCAGCCCCTGCCAGCAATGGGGATTGAATCGCGAAGTTACACC
CGTCTTTGGTGCGCGTCTGGTGCAGGAAGGCAATCGTCTGCATTTTCTGACTGATCGTGCTGGGTTTAATGGTGCATTCA
GTGAGGATGACGCGCTACGGCTCGACCAGGCATTTCCGCTGATGCTCAAACAGCTTGAAATTGAGCTGCGCTCAGGTGAG
CTCAATCCTCGTCGTCAACATTGCGTTACGTTGAACCACAATGGCCTGACCTGCGAAGCCGACACCCTCGGTTCTTACGG
GTATGTTTATATTGCCATTTACCCGACCAGCCTGTGCTCACCTGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G5KEF9