Detailed information of TA system TA110055

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4061836..4062592
• Replicon: chromosome
• Accession: NZ_CP031780
• Organism: Yersinia pseudotuberculosis strain FDAARGOS_342

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A3G5KDU1
• Locus tag: CEQ20_RS18935
• Protein ID: WP_032467110.1
• Coordinates: 4062224..4062592 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: Q664A5
• Locus tag: CEQ20_RS18930
• Protein ID: WP_011193303.1
• Coordinates: 4061836..4062171 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ20_RS18900	4057530..4057823	+	294	Protein_3511	ATP-binding protein	-
CEQ20_RS18905	4057877..4058512	+	636	WP_098087291.1	hypothetical protein	-
CEQ20_RS18910	4058887..4059974	+	1088	WP_098087213.1	IS3 family transposase	-
CEQ20_RS18915	4059999..4060514	+	516	WP_050321080.1	hypothetical protein	-
CEQ20_RS18920	4060542..4061162	+	621	WP_011193305.1	hypothetical protein	-
CEQ20_RS18925	4061345..4061824	+	480	WP_011193304.1	hypothetical protein	-
CEQ20_RS18930	4061836..4062171	+	336	WP_011193303.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CEQ20_RS18935	4062224..4062592	+	369	WP_032467110.1	TA system toxin CbtA family protein	Toxin
CEQ20_RS18940	4062819..4063673	+	855	WP_032467109.1	DUF4942 domain-containing protein	-
CEQ20_RS18945	4064036..4064308	+	273	WP_011193300.1	Arm DNA-binding domain-containing protein	-
CEQ20_RS18950	4064543..4064950	+	408	WP_011193299.1	hypothetical protein	-
CEQ20_RS18955	4064973..4065299	+	327	WP_011193298.1	hypothetical protein	-
CEQ20_RS18960	4065241..4065741	+	501	WP_050117508.1	virulence RhuM family protein	-
CEQ20_RS18965	4065738..4066496	-	759	WP_002209584.1	ABC transporter ATP-binding protein	-
CEQ20_RS18970	4066493..4067500	-	1008	WP_011193296.1	iron chelate uptake ABC transporter family permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13723.81 Da
• Isoelectric Point: 6.4530

>T110055 WP_032467110.1 NZ_CP031780:4062224-4062592 [Yersinia pseudotuberculosis]
MQILPVTPKRAAYACPSPVVVWQSMLTYLLEQHYGLALNDTEFSDDAVIHEYIDAGISLSDALNFTVEKFGLVRIDRRGF
SCQEQSPFITAIDILRARRATGLMTRLGYQTIISVIRGEKQT

Nucleotide

• Length: 369 bp

>T110055 NZ_CP031780:4062224-4062592 [Yersinia pseudotuberculosis]
ATGCAGATCTTACCTGTCACTCCGAAACGGGCGGCTTATGCTTGCCCATCACCCGTTGTTGTCTGGCAATCCATGCTCAC
GTATCTGCTGGAACAGCATTACGGATTGGCTCTTAACGATACCGAGTTCAGTGATGACGCCGTGATCCACGAATATATTG
ATGCTGGTATCTCTCTGTCCGATGCGTTGAATTTCACGGTGGAAAAGTTTGGCCTGGTTCGTATCGATCGCCGGGGGTTC
TCCTGCCAGGAACAATCTCCCTTCATCACGGCTATCGATATTCTCCGTGCCCGTCGAGCCACCGGCCTGATGACCCGTCT
GGGTTACCAGACCATCATCTCAGTTATCCGGGGCGAAAAGCAGACATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12452.06 Da
• Isoelectric Point: 6.0555

>AT110055 WP_011193303.1 NZ_CP031780:4061836-4062171 [Yersinia pseudotuberculosis]
MPSITTPAWGLKRNVTPQFGARLVQEGDRLHFLADRADINGTFSEVQVRDLDNAFPQFINYLELMLLSGELNPRHQHCVT
LYRNGLTCEADSLGSHGYVYLAIYPTPQSTA

Nucleotide

• Length: 336 bp

>AT110055 NZ_CP031780:4061836-4062171 [Yersinia pseudotuberculosis]
ATGCCATCAATCACTACCCCAGCCTGGGGACTTAAACGCAACGTCACTCCGCAGTTTGGTGCCCGTCTGGTACAGGAAGG
CGACCGACTACATTTTCTAGCCGACCGTGCCGATATCAACGGAACCTTCAGCGAAGTGCAGGTACGTGATTTAGATAACG
CCTTCCCGCAGTTCATCAACTACCTGGAACTGATGCTGCTCTCCGGTGAGCTCAACCCTCGCCACCAGCACTGTGTCACG
TTGTATCGCAATGGCTTGACCTGTGAGGCCGATAGCCTGGGATCACACGGTTATGTGTATCTCGCTATTTATCCTACTCC
CCAATCAACTGCATAA

Structures

Toxin

• AlphaFold DB: A0A3G5KDU1

Antitoxin

• AlphaFold DB: Q664A5