Detailed information of TA system TA110034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2124249..2124763
• Replicon: chromosome
• Accession: NZ_CP031779
• Organism: Staphylococcus aureus strain CFBR-105

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: D1F65_RS10830
• Protein ID: WP_000581792.1
• Coordinates: 2124249..2124458 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: D1F65_RS10835
• Protein ID: WP_000138932.1
• Coordinates: 2124470..2124763 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1F65_RS10810	2119757..2121115	-	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
D1F65_RS10815	2121130..2122200	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
D1F65_RS10820	2122518..2123720	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
D1F65_RS10825	2123984..2124121	-	138	WP_000828354.1	hypothetical protein	-
D1F65_RS10830	2124249..2124458	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
D1F65_RS10835	2124470..2124763	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
D1F65_RS10840	2124929..2126413	+	1485	WP_000571549.1	cardiolipin synthase	-
D1F65_RS10845	2126441..2127088	+	648	WP_001187630.1	HD domain-containing protein	-
D1F65_RS10870	2128001..2128873	-	873	WP_000725802.1	membrane protein insertase YidC	-
D1F65_RS10875	2128959..2129600	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T110034 WP_000581792.1 NZ_CP031779:c2124458-2124249 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T110034 NZ_CP031779:c2124458-2124249 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT110034 WP_000138932.1 NZ_CP031779:c2124763-2124470 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT110034 NZ_CP031779:c2124763-2124470 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13