Detailed information of TA system TA110033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102990..2103519
• Replicon: chromosome
• Accession: NZ_CP031779
• Organism: Staphylococcus aureus strain CFBR-105

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D1F65_RS10730
• Protein ID: WP_000621175.1
• Coordinates: 2102990..2103352 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: D1F65_RS10735
• Protein ID: WP_000948331.1
• Coordinates: 2103349..2103519 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1F65_RS10705	2099968..2100738	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
D1F65_RS10710	2100713..2101192	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
D1F65_RS10715	2101194..2101520	-	327	WP_001052491.1	anti-sigma factor antagonist	-
D1F65_RS10720	2101639..2102640	-	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
D1F65_RS10730	2102990..2103352	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D1F65_RS10735	2103349..2103519	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
D1F65_RS10740	2103604..2104752	-	1149	WP_001281154.1	alanine racemase	-
D1F65_RS10745	2104818..2105177	-	360	WP_000581197.1	holo-ACP synthase	-
D1F65_RS10750	2105181..2105672	-	492	WP_001205912.1	PH domain-containing protein	-
D1F65_RS10755	2105659..2107242	-	1584	WP_001294626.1	PH domain-containing protein	-
D1F65_RS10760	2107235..2107714	-	480	WP_001287087.1	hypothetical protein	-
D1F65_RS10765	2107922..2108482	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T110033 WP_000621175.1 NZ_CP031779:c2103352-2102990 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T110033 NZ_CP031779:c2103352-2102990 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT110033 WP_000948331.1 NZ_CP031779:c2103519-2103349 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT110033 NZ_CP031779:c2103519-2103349 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1