Detailed information of TA system TA110026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 2320944..2321503
• Replicon: chromosome
• Accession: NZ_CP031775
• Organism: Shewanella decolorationis strain Ni1-3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: D0436_RS10040
• Protein ID: WP_172591132.1
• Coordinates: 2321114..2321503 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: D0436_RS10035
• Protein ID: WP_172591131.1
• Coordinates: 2320944..2321114 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0436_RS10020 (D0436_12320)	2316565..2318559	+	1995	WP_133181228.1	nitrate- and nitrite sensing domain-containing protein	-
D0436_RS10030 (D0436_12310)	2318990..2320813	+	1824	WP_254803640.1	tyrosine-type recombinase/integrase	-
D0436_RS10035 (D0436_12305)	2320944..2321114	+	171	WP_172591131.1	acetyltransferase	Antitoxin
D0436_RS10040 (D0436_12300)	2321114..2321503	+	390	WP_172591132.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
D0436_RS10045 (D0436_12295)	2321630..2324356	-	2727	WP_208658946.1	phage/plasmid primase, P4 family	-
D0436_RS10050 (D0436_12290)	2324499..2324822	-	324	WP_208658944.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14212.28 Da
• Isoelectric Point: 3.9777

>T110026 WP_172591132.1 NZ_CP031775:2321114-2321503 [Shewanella decolorationis]
MDIICFPFERVVEINALILSTEPGMKGAVDIPKLQGALSRIDNAIVYQGLDDVFEIAAKYTACIAVSHACPDANKRTGLA
VALEYLSLNDYEITEDNELLANAIRDLVLQEITETDFADILYAQYLKGL

Nucleotide

• Length: 390 bp

>T110026 NZ_CP031775:2321114-2321503 [Shewanella decolorationis]
ATGGATATCATCTGTTTTCCTTTTGAACGTGTGGTCGAAATTAATGCGTTAATCCTTTCGACAGAGCCCGGCATGAAAGG
TGCGGTTGATATTCCTAAACTTCAGGGAGCGTTGAGCCGTATTGACAATGCGATTGTCTACCAAGGCTTAGATGATGTGT
TTGAGATTGCTGCAAAATACACTGCTTGTATAGCGGTATCTCACGCTTGTCCTGATGCAAATAAGCGAACTGGTTTAGCA
GTCGCACTCGAATACTTATCTCTTAACGACTATGAAATCACAGAGGATAATGAACTGCTAGCGAATGCTATCCGAGACTT
GGTGCTTCAAGAGATTACCGAGACTGACTTTGCTGACATTCTATATGCTCAGTATCTCAAGGGGTTGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6414.58 Da
• Isoelectric Point: 11.0174

>AT110026 WP_172591131.1 NZ_CP031775:2320944-2321114 [Shewanella decolorationis]
MNRKLEVYGVQVVTRPKIKATKQLDLSGDFGRQIVKSETKLALRTHPKTFQKLADM

Nucleotide

• Length: 171 bp

>AT110026 NZ_CP031775:2320944-2321114 [Shewanella decolorationis]
ATGAATCGAAAATTAGAAGTTTACGGTGTTCAAGTGGTAACTAGACCGAAGATCAAGGCGACCAAACAACTTGATTTATC
AGGTGACTTTGGACGTCAAATCGTTAAGTCAGAAACCAAACTAGCATTAAGAACCCATCCCAAAACTTTCCAAAAGTTGG
CTGATATGTAA