Detailed information of TA system TA110022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelB(antitoxin)
• Location: 1658477..1659089
• Replicon: chromosome
• Accession: NZ_CP031770
• Organism: Streptococcus pyogenes strain SASM4-Duke

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q1JJZ2
• Locus tag: D1F63_RS08620
• Protein ID: WP_002988077.1
• Coordinates: 1658754..1659089 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5X9X9
• Locus tag: D1F63_RS08615
• Protein ID: WP_002988079.1
• Coordinates: 1658477..1658764 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1F63_RS08590	1653666..1654649	-	984	WP_011529001.1	tagatose-bisphosphate aldolase	-
D1F63_RS08595	1654653..1655582	-	930	WP_011529002.1	tagatose-6-phosphate kinase	-
D1F63_RS08600	1655630..1656145	-	516	WP_011529003.1	galactose-6-phosphate isomerase subunit LacB	-
D1F63_RS08605	1656180..1656608	-	429	WP_011529004.1	galactose-6-phosphate isomerase subunit LacA	-
D1F63_RS08610	1657054..1657827	+	774	WP_011529005.1	DeoR/GlpR family DNA-binding transcription regulator	-
D1F63_RS08615	1658477..1658764	+	288	WP_002988079.1	DNA-damage-inducible protein J	Antitoxin
D1F63_RS08620	1658754..1659089	+	336	WP_002988077.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1F63_RS08625	1659228..1659404	+	177	Protein_1588	tyrosine-type recombinase/integrase	-
D1F63_RS08635	1659535..1659927	-	393	WP_002982716.1	30S ribosomal protein S9	-
D1F63_RS08640	1659948..1660394	-	447	WP_002982710.1	50S ribosomal protein L13	-
D1F63_RS08645	1660612..1660818	-	207	WP_011529007.1	helix-turn-helix transcriptional regulator	-
D1F63_RS08650	1660815..1661513	-	699	WP_021299047.1	hypothetical protein	-
D1F63_RS08655	1661649..1662509	-	861	WP_011529009.1	DegV family protein	-
D1F63_RS08660	1662606..1663124	-	519	WP_011055010.1	NYN domain-containing protein	-
D1F63_RS08665	1663128..1663874	-	747	WP_010922667.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-105)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13117.83 Da
• Isoelectric Point: 5.2144

>T110022 WP_002988077.1 NZ_CP031770:1658754-1659089 [Streptococcus pyogenes]
MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISDIEKLEVFPEVGFDADEKYGSKISHYHSTKGYTLSKD
YIVLYHIEGEENRVAIDYLLPTQSDYIKLFK

Nucleotide

• Length: 336 bp

>T110022 NZ_CP031770:1658754-1659089 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTACTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGCCATTATCACAGCACTAAAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGCTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10934.56 Da
• Isoelectric Point: 5.2318

>AT110022 WP_002988079.1 NZ_CP031770:1658477-1658764 [Streptococcus pyogenes]
MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQ
GKFYTSEEVRAELGL

Nucleotide

• Length: 288 bp

>AT110022 NZ_CP031770:1658477-1658764 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGACGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Toxin

• AlphaFold DB: A0A660A236

Antitoxin

• AlphaFold DB: A0A4U7GX94