Detailed information of TA system TA109997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1659494..1660515
• Replicon: chromosome
• Accession: NZ_CP031756
• Organism: Klebsiella aerogenes strain FDAARGOS_327

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A0G9UXE3
• Locus tag: CEQ05_RS08415
• Protein ID: WP_032706547.1
• Coordinates: 1659494..1660069 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0G9R618
• Locus tag: CEQ05_RS08420
• Protein ID: WP_015369110.1
• Coordinates: 1660069..1660515 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ05_RS08400	1656779..1657816	+	1038	WP_032706544.1	virulence RhuM family protein	-
CEQ05_RS08405	1657993..1659189	-	1197	WP_015369112.1	hypothetical protein	-
CEQ05_RS08415	1659494..1660069	-	576	WP_032706547.1	PIN domain-containing protein	Toxin
CEQ05_RS08420	1660069..1660515	-	447	WP_015369110.1	helix-turn-helix domain-containing protein	Antitoxin
CEQ05_RS08425	1660726..1661733	+	1008	WP_015369109.1	zinc-binding alcohol dehydrogenase family protein	-
CEQ05_RS08430	1661734..1662036	+	303	WP_015369108.1	antibiotic biosynthesis monooxygenase	-
CEQ05_RS08435	1662194..1663243	+	1050	WP_015369107.1	NAD(P)-dependent alcohol dehydrogenase	-
CEQ05_RS08440	1663240..1664193	-	954	WP_032706577.1	helix-turn-helix domain-containing protein	-
CEQ05_RS08445	1664215..1664994	-	780	WP_015369105.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (79-126)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21688.29 Da
• Isoelectric Point: 6.2900

>T109997 WP_032706547.1 NZ_CP031756:c1660069-1659494 [Klebsiella aerogenes]
MRHSPYPVILDACVLYPARLRDLLMHLGIAGLYQPKWSRFIHDEWCRNLLINRPDIAPEALRRTVDLMNAALPDANVTGF
AGLINGLVLPDPDDRHVLAAAIRAKAEIIVTLNHKDFPAESLLPFEIETLHPDVFISDLFDLNHALALEAVRRQRQSLKH
PPMTVDEFLEMLLKQGLPMTVKELANYQYAI

Nucleotide

• Length: 576 bp

>T109997 NZ_CP031756:c1660069-1659494 [Klebsiella aerogenes]
ATGCGACATTCACCTTATCCGGTGATTTTAGATGCTTGTGTATTATATCCAGCACGGCTCAGGGATTTATTAATGCACTT
AGGGATCGCTGGACTTTATCAACCAAAATGGAGCCGCTTCATTCACGATGAATGGTGCAGGAATCTTCTTATAAATCGGC
CTGATATAGCACCTGAAGCGCTGCGTCGTACCGTTGATCTCATGAATGCAGCTTTACCGGATGCTAATGTAACGGGTTTT
GCAGGATTAATTAACGGACTAGTTTTACCCGACCCTGACGATCGGCATGTCCTTGCTGCGGCAATCAGAGCGAAGGCTGA
AATTATCGTTACGTTAAATCATAAAGACTTTCCAGCAGAATCATTGCTTCCATTTGAAATCGAAACACTTCATCCTGATG
TATTCATATCGGACCTGTTTGACCTTAATCATGCTCTGGCGTTGGAGGCGGTGAGAAGGCAGCGGCAGTCTCTCAAGCAT
CCTCCAATGACCGTCGATGAGTTCCTGGAGATGCTGCTAAAGCAGGGCTTGCCGATGACGGTAAAAGAGTTAGCCAATTA
CCAGTACGCCATATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16699.26 Da
• Isoelectric Point: 6.2257

>AT109997 WP_015369110.1 NZ_CP031756:c1660515-1660069 [Klebsiella aerogenes]
MTKLNLPDPLESELAQRGQRELAAYLSTHLETQRIAIVGEDDKTHTIELPTSALTMLMEILGELACGNAVQIVPVHAELT
TQEAANILNVSRPHMVKLLEARKLPFHKTGRHRRVLFADLMEYKKRREQESLDAMQALADQAQDLGMY

Nucleotide

• Length: 447 bp

>AT109997 NZ_CP031756:c1660515-1660069 [Klebsiella aerogenes]
ATGACTAAACTGAACCTTCCGGACCCGCTGGAGAGTGAACTTGCCCAGCGCGGGCAGCGTGAGCTGGCGGCATACCTTTC
TACTCATCTTGAAACGCAGCGTATTGCGATTGTGGGTGAAGATGACAAAACTCATACCATTGAGTTGCCAACCAGCGCTC
TGACTATGCTGATGGAGATCCTTGGGGAATTGGCTTGTGGCAACGCGGTGCAGATTGTTCCTGTTCATGCTGAACTCACC
ACCCAGGAAGCAGCGAATATACTCAATGTTTCTCGTCCGCATATGGTTAAACTGCTGGAAGCGAGGAAGCTTCCATTTCA
TAAAACTGGACGCCATCGCCGGGTTCTGTTTGCCGATCTGATGGAGTATAAAAAACGTAGGGAACAGGAAAGCCTGGACG
CGATGCAAGCGCTGGCGGATCAGGCGCAGGACTTAGGGATGTATTAA

Structures

Toxin

• AlphaFold DB: A0A0G9UXE3

Antitoxin

• AlphaFold DB: A0A0G9R618