Detailed information of TA system TA109993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 11367..11983
• Replicon: plasmid p.C
• Accession: NZ_CP031754
• Organism: Cereibacter sphaeroides strain EBL0706

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D0Z66_RS21585
• Protein ID: WP_108222584.1
• Coordinates: 11367..11774 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D0Z66_RS21590
• Protein ID: WP_181318578.1
• Coordinates: 11774..11983 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0Z66_RS21560	6900..7679	+	780	WP_108222589.1	recombinase family protein	-
D0Z66_RS21565	7980..8381	-	402	WP_108222588.1	hypothetical protein	-
D0Z66_RS21570	8400..8663	-	264	WP_108222587.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
D0Z66_RS22305	8744..8908	-	165	WP_181318579.1	hypothetical protein	-
D0Z66_RS21575	9091..9399	+	309	WP_108222586.1	hypothetical protein	-
D0Z66_RS21580	9917..11080	-	1164	WP_108222585.1	DUF4268 domain-containing protein	-
D0Z66_RS21585	11367..11774	-	408	WP_108222584.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D0Z66_RS21590	11774..11983	-	210	WP_181318578.1	antitoxin	Antitoxin
D0Z66_RS21595	12175..12702	+	528	WP_108222583.1	hypothetical protein	-
D0Z66_RS21600	12866..14647	+	1782	WP_108222582.1	DUF927 domain-containing protein	-
D0Z66_RS21605	14969..15133	+	165	Protein_16	IS5/IS1182 family transposase	-
D0Z66_RS22060	15532..16680	+	1149	WP_146172339.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..43846	43846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14941.25 Da
• Isoelectric Point: 7.3602

>T109993 WP_108222584.1 NZ_CP031754:c11774-11367 [Cereibacter sphaeroides]
MLRYMLDTNILIYTIRNRPGEVRAKFEAHHGTMCISTVTAMELIFGAQKSQAVERNMAAVEGLLARLDILDLDLAAAQHA
GEIRAQLARAGTPIGPFDVMIAGHARSRGLVLVSNNTREFERVDGLRLENWVQPS

Nucleotide

• Length: 408 bp

>T109993 NZ_CP031754:c11774-11367 [Cereibacter sphaeroides]
ATGCTGCGCTACATGCTCGACACGAACATCCTGATCTACACGATCAGGAACCGGCCTGGCGAGGTCAGGGCGAAATTCGA
GGCCCACCATGGGACGATGTGCATCTCGACCGTAACCGCGATGGAACTGATCTTCGGCGCGCAGAAATCGCAGGCCGTCG
AACGCAACATGGCGGCGGTCGAGGGACTTCTTGCGCGCCTCGACATCCTGGATCTCGACTTGGCGGCTGCCCAGCACGCT
GGGGAGATCCGGGCGCAGTTGGCGAGGGCAGGGACGCCCATCGGACCGTTCGACGTGATGATCGCCGGACACGCGCGATC
GAGGGGCCTGGTCCTCGTGAGCAACAACACGCGGGAATTCGAGCGCGTCGACGGCCTGAGGCTTGAGAACTGGGTCCAGC
CGTCCTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8017.86 Da
• Isoelectric Point: 4.2683

>AT109993 WP_181318578.1 NZ_CP031754:c11983-11774 [Cereibacter sphaeroides]
MNNRTQAIRLPKSVALPEGTRSVDIVRLGRAWLIVPSQDSWAQWFDEVSSSEDFMERREQPDADERDDL

Nucleotide

• Length: 210 bp

>AT109993 NZ_CP031754:c11983-11774 [Cereibacter sphaeroides]
ATGAACAACCGGACGCAGGCCATCCGCCTGCCGAAATCGGTTGCCCTGCCGGAAGGCACGCGGAGCGTCGACATAGTCCG
GCTGGGCAGGGCTTGGCTCATCGTGCCGAGCCAGGACAGTTGGGCTCAGTGGTTCGATGAGGTCTCGTCGAGTGAAGACT
TCATGGAGCGCCGGGAGCAACCGGACGCTGATGAACGCGACGATCTCTGA