Detailed information of TA system TA109959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 37143..37940
• Replicon: plasmid p1_020038
• Accession: NZ_CP031722
• Organism: Enterobacter hormaechei strain WCHEH020038

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: D0Z05_RS00260
• Protein ID: WP_172754447.1
• Coordinates: 37437..37940 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: D0Z05_RS00255
• Protein ID: WP_016582626.1
• Coordinates: 37143..37412 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0Z05_RS00230	32473..33813	-	1341	WP_006812582.1	AAA family ATPase	-
D0Z05_RS00235	33874..34599	-	726	WP_058652463.1	hypothetical protein	-
D0Z05_RS00240	34863..35660	+	798	WP_077990606.1	DUF2971 domain-containing protein	-
D0Z05_RS00245	35702..36055	-	354	WP_160956455.1	hypothetical protein	-
D0Z05_RS00250	36061..36726	-	666	WP_023315962.1	AAA family ATPase	-
D0Z05_RS00255	37143..37412	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
D0Z05_RS00260	37437..37940	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
D0Z05_RS25505	37968..38174	-	207	WP_080399007.1	hypothetical protein	-
D0Z05_RS25510	38125..38376	-	252	WP_000147960.1	hypothetical protein	-
D0Z05_RS00270	38378..39070	-	693	WP_006812500.1	membrane protein	-
D0Z05_RS00275	39084..39407	-	324	WP_000064173.1	hypothetical protein	-
D0Z05_RS00280	39482..40270	-	789	WP_040110304.1	receptor-recognizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114700	114700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T109959 WP_172754447.1 NZ_CP031722:37437-37940 [Enterobacter hormaechei]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T109959 NZ_CP031722:37437-37940 [Enterobacter hormaechei]
ATGTTTTCAGCACAAGCCGTATACGATTTTTCGGGCTTCGACTGCGGCGAACCATCTCTGAATGATTTCCTGCAAAACCG
CCTGGTACAGCAACACAACGGGCGTATATTACGTGGCTACCTGCTTCTTACCAAAGATCCGGTGCCGAAAGTTAAGGGTT
TTTACACGCTATCCGGGAGCTGTTTTGCAAGACAGACGCTACCATCGAACACCCAGAAGCGGAAAATCCCCTACGCGGAT
GCCCCCAGCGTTACGCTTGGCCGTCTGGCAATAGATATCAGCCTACAGAAACAAGGCTACGGGGAGGTTCTCGTAACGCA
CGCGTTGAAGGTTGTTTATCAGGCTTCTCGCGCAGTAGGCATATACGCTTTGTTTGTGGATGCACTAAACCAAAGCGCAA
TGCAGTTCTATCAGAAGTTTGGATTCATCCCTTTGACGGGGGCAAATGCCAACTCGCTTTTCTACCCCACGAAAAGCATT
GAAGAGCTGTTTGAAACAGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT109959 WP_016582626.1 NZ_CP031722:37143-37412 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT109959 NZ_CP031722:37143-37412 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACGATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGCT
TGATTCTCAGTGAAGAGTCCTGGAATCTGGTAATGGATGCCATCAGTAACCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAACGTCTACAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3