Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 113076..113719 Replicon plasmid pKPC2_020003
Accession NZ_CP031720
Organism Klebsiella pneumoniae strain SCKP020003

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CAY64_RS01040 Protein ID WP_001044770.1
Coordinates 113076..113492 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CAY64_RS01045 Protein ID WP_001261282.1
Coordinates 113489..113719 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY64_RS01020 109179..109451 - 273 Protein_135 transposase -
CAY64_RS01025 109480..110177 - 698 Protein_136 IS1 family transposase -
CAY64_RS01030 110433..111455 - 1023 WP_000361404.1 helicase UvrD -
CAY64_RS01035 111440..113002 - 1563 WP_004206609.1 AAA family ATPase -
CAY64_RS01040 113076..113492 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CAY64_RS01045 113489..113719 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CAY64_RS01050 113676..114137 + 462 WP_014343465.1 hypothetical protein -
CAY64_RS01055 114298..115242 + 945 WP_011977810.1 hypothetical protein -
CAY64_RS01060 115279..115671 + 393 WP_011977811.1 hypothetical protein -
CAY64_RS01065 115729..116250 + 522 WP_013214008.1 hypothetical protein -
CAY64_RS01070 116296..116499 + 204 WP_011977813.1 hypothetical protein -
CAY64_RS01075 116529..117533 + 1005 WP_011977814.1 hypothetical protein -
CAY64_RS01080 117717..118496 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 - 1..154957 154957


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T109940 WP_001044770.1 NZ_CP031720:c113492-113076 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T109940 NZ_CP031720:c113492-113076 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT109940 WP_001261282.1 NZ_CP031720:c113719-113489 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT109940 NZ_CP031720:c113719-113489 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References