Detailed information of TA system TA109931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 799..1273
• Replicon: plasmid p3_020003
• Accession: NZ_CP031719
• Organism: Klebsiella pneumoniae strain SCKP020003

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: CAY64_RS00215
• Protein ID: WP_004197762.1
• Coordinates: 799..1065 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: CAY64_RS00220
• Protein ID: WP_000879771.1
• Coordinates: 1049..1273 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY64_RS00210	379..729	+	351	WP_004197756.1	hypothetical protein	-
CAY64_RS00215	799..1065	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CAY64_RS00220	1049..1273	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
CAY64_RS00225	1940..2152	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
CAY64_RS00230	2159..2668	-	510	WP_032433972.1	MbeB family mobilization protein	-
CAY64_RS00235	2643..3407	-	765	Protein_6	relaxase/mobilization nuclease domain-containing protein	-
CAY64_RS00240	3346..3690	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
CAY64_RS29155	3680..3856	-	177	WP_004197757.1	hypothetical protein	-
CAY64_RS00250	4959..5338	+	380	Protein_9	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T109931 WP_004197762.1 NZ_CP031719:c1065-799 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T109931 NZ_CP031719:c1065-799 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT109931 WP_000879771.1 NZ_CP031719:c1273-1049 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT109931 NZ_CP031719:c1273-1049 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5