Detailed information of TA system TA109922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 25984..26572
• Replicon: plasmid p2_010060
• Accession: NZ_CP031710
• Organism: Acinetobacter wuhouensis strain WCHA60

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A385C154
• Locus tag: BEN71_RS00375
• Protein ID: WP_068975324.1
• Coordinates: 25984..26271 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A385C0S9
• Locus tag: BEN71_RS00380
• Protein ID: WP_068975325.1
• Coordinates: 26258..26572 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN71_RS00350	21118..22593	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
BEN71_RS00355	22649..23533	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
BEN71_RS00360	23723..24334	-	612	WP_015060246.1	recombinase family protein	-
BEN71_RS00365	24531..25256	-	726	WP_068975322.1	hypothetical protein	-
BEN71_RS00375	25984..26271	+	288	WP_068975324.1	BrnT family toxin	Toxin
BEN71_RS00380	26258..26572	+	315	WP_068975325.1	BrnA antitoxin family protein	Antitoxin
BEN71_RS00385	26663..26860	+	198	Protein_34	hypothetical protein	-
BEN71_RS00395	28281..28565	-	285	WP_086322806.1	hypothetical protein	-
BEN71_RS00400	29197..29400	-	204	WP_068975480.1	hypothetical protein	-
BEN71_RS00405	29427..29759	-	333	WP_068975481.1	type II toxin-antitoxin system YafQ family toxin	-
BEN71_RS00410	29731..30012	-	282	WP_068975482.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E)	-	1..30787	30787
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	21118..28026	6908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11276.69 Da
• Isoelectric Point: 6.0884

>T109922 WP_068975324.1 NZ_CP031710:25984-26271 [Acinetobacter wuhouensis]
MKQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T109922 NZ_CP031710:25984-26271 [Acinetobacter wuhouensis]
ATGAAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGATGATCCACTGAGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAGACCATTGGGA
AAGTAAAAGGCGTACTAATGCTGTTAGTAGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTCACTAAGGCGGAGCGAGATAGATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12268.89 Da
• Isoelectric Point: 6.4768

>AT109922 WP_068975325.1 NZ_CP031710:26258-26572 [Acinetobacter wuhouensis]
MSMVRYSRKELNEKFSDKQDAEIERLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILREAMLEDLKKHQ

Nucleotide

• Length: 315 bp

>AT109922 NZ_CP031710:26258-26572 [Acinetobacter wuhouensis]
ATGAGCATGGTTAGATACTCACGTAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTGAACGCTTGCT
TGCTAAAGGGACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATCCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCACAG
GGAAAAGGTTATCAAACACGAATGAATGAAATTCTGCGAGAAGCAATGTTAGAAGATTTAAAAAAACATCAATAA

Structures

Toxin

• AlphaFold DB: A0A385C154

Antitoxin

• AlphaFold DB: A0A385C0S9