Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4505553..4506184 Replicon chromosome
Accession NZ_CP031706
Organism Escherichia coli strain R1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag D0T88_RS21800 Protein ID WP_001259385.1
Coordinates 4505909..4506184 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag D0T88_RS21795 Protein ID WP_000593554.1
Coordinates 4505553..4505912 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0T88_RS21765 4500665..4501138 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
D0T88_RS21770 4501135..4501416 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
D0T88_RS21775 4501493..4502851 + 1359 WP_063113578.1 PTS galactitol transporter subunit IIC -
D0T88_RS21780 4502844..4504352 + 1509 WP_001298718.1 carbohydrate kinase -
D0T88_RS21785 4504342..4504611 + 270 WP_000933060.1 HPr family phosphocarrier protein -
D0T88_RS21790 4504643..4505503 + 861 WP_001131783.1 class II aldolase -
D0T88_RS21795 4505553..4505912 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
D0T88_RS21800 4505909..4506184 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
D0T88_RS21805 4506257..4507381 - 1125 WP_001216257.1 ABC transporter permease -
D0T88_RS21810 4507381..4510116 - 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
D0T88_RS21815 4510113..4511180 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T109913 WP_001259385.1 NZ_CP031706:c4506184-4505909 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T109913 NZ_CP031706:c4506184-4505909 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT109913 WP_000593554.1 NZ_CP031706:c4505912-4505553 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT109913 NZ_CP031706:c4505912-4505553 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References