Detailed information of TA system TA109909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3452859..3453484
• Replicon: chromosome
• Accession: NZ_CP031706
• Organism: Escherichia coli strain R1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D0T88_RS16630
• Protein ID: WP_000911330.1
• Coordinates: 3452859..3453257 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: D0T88_RS16635
• Protein ID: WP_000450524.1
• Coordinates: 3453257..3453484 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0T88_RS16610	3448766..3448966	+	201	WP_000383836.1	YpfN family protein	-
D0T88_RS16615	3449047..3449745	-	699	WP_000679812.1	esterase	-
D0T88_RS16620	3449819..3451834	-	2016	WP_033885019.1	tRNA cytosine(34) acetyltransferase TmcA	-
D0T88_RS16625	3451849..3452712	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
D0T88_RS16630	3452859..3453257	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D0T88_RS16635	3453257..3453484	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
D0T88_RS16640	3453638..3454351	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
D0T88_RS16645	3454564..3455598	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
D0T88_RS16650	3455615..3456493	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
D0T88_RS16655	3456639..3457211	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
D0T88_RS16660	3457211..3457681	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T109909 WP_000911330.1 NZ_CP031706:c3453257-3452859 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T109909 NZ_CP031706:c3453257-3452859 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT109909 WP_000450524.1 NZ_CP031706:c3453484-3453257 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT109909 NZ_CP031706:c3453484-3453257 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33