Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2149102..2149740 Replicon chromosome
Accession NZ_CP031706
Organism Escherichia coli strain R1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag D0T88_RS10330 Protein ID WP_000813794.1
Coordinates 2149102..2149278 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag D0T88_RS10335 Protein ID WP_001270286.1
Coordinates 2149324..2149740 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0T88_RS10310 2144721..2145935 - 1215 WP_074147490.1 BenE family transporter YdcO -
D0T88_RS10315 2145988..2146524 + 537 WP_063103683.1 DNA-binding transcriptional regulator SutR -
D0T88_RS10320 2146597..2148558 + 1962 WP_024179169.1 23S rRNA 5-hydroxycytidine C2501 synthase -
D0T88_RS10325 2148650..2148880 - 231 WP_000494244.1 YncJ family protein -
D0T88_RS10330 2149102..2149278 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
D0T88_RS10335 2149324..2149740 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
D0T88_RS10340 2149819..2151225 + 1407 WP_063113709.1 PLP-dependent aminotransferase family protein -
D0T88_RS10345 2151470..2152615 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
D0T88_RS10350 2152633..2153643 + 1011 Protein_2016 ABC transporter ATP-binding protein -
D0T88_RS10355 2153647..2154588 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2143416..2144681 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T109898 WP_000813794.1 NZ_CP031706:2149102-2149278 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T109898 NZ_CP031706:2149102-2149278 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT109898 WP_001270286.1 NZ_CP031706:2149324-2149740 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT109898 NZ_CP031706:2149324-2149740 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References