Detailed information of TA system TA109894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1045841..1046678
• Replicon: chromosome
• Accession: NZ_CP031706
• Organism: Escherichia coli strain R1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: D0T88_RS04915
• Protein ID: WP_000227784.1
• Coordinates: 1045841..1046383 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: D0T88_RS04920
• Protein ID: WP_001297137.1
• Coordinates: 1046367..1046678 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0T88_RS04895	1042143..1042523	+	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
D0T88_RS04900	1042520..1042867	+	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
D0T88_RS04905	1042917..1044455	+	1539	WP_137546056.1	IS66-like element ISEc8 family transposase	-
D0T88_RS04910	1044850..1045785	+	936	WP_001297127.1	sel1 repeat family protein	-
D0T88_RS04915	1045841..1046383	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
D0T88_RS04920	1046367..1046678	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
D0T88_RS04925	1046863..1047753	-	891	WP_000971336.1	heme o synthase	-
D0T88_RS04930	1047765..1048094	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
D0T88_RS04935	1048094..1048708	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
D0T88_RS04940	1048698..1050689	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
D0T88_RS04945	1050711..1051658	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T109894 WP_000227784.1 NZ_CP031706:c1046383-1045841 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T109894 NZ_CP031706:c1046383-1045841 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT109894 WP_001297137.1 NZ_CP031706:c1046678-1046367 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT109894 NZ_CP031706:c1046678-1046367 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3