Detailed information of TA system TA109884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3348966..3349711
• Replicon: chromosome
• Accession: NZ_CP031698
• Organism: Xanthomonas oryzae pv. oryzae strain PXO142

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: DZA55_RS16500
• Protein ID: WP_199285672.1
• Coordinates: 3348966..3349295 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: DZA55_RS16505
• Protein ID: WP_011407754.1
• Coordinates: 3349292..3349711 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZA55_RS16470 (DZA55_16460)	3344099..3344770	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
DZA55_RS16480 (DZA55_16470)	3345071..3345862	-	792	WP_011407758.1	polysaccharide deacetylase family protein	-
DZA55_RS16485 (DZA55_16475)	3345908..3346834	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
DZA55_RS16490 (DZA55_16480)	3347166..3347501	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
DZA55_RS16495 (DZA55_16485)	3347545..3348863	-	1319	Protein_2988	IS701 family transposase	-
DZA55_RS16500 (DZA55_16490)	3348966..3349295	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
DZA55_RS16505 (DZA55_16495)	3349292..3349711	+	420	WP_011407754.1	macro domain-containing protein	Antitoxin
DZA55_RS16515 (DZA55_16505)	3349763..3350731	+	969	WP_011407317.1	IS5-like element ISXo1 family transposase	-
DZA55_RS16520 (DZA55_16510)	3350842..3351522	+	681	WP_011257926.1	phosphate phosphatase	-
DZA55_RS16525 (DZA55_16515)	3351560..3352795	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
DZA55_RS16530 (DZA55_16520)	3353097..3353423	+	327	Protein_2994	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3347475..3352795	5320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T109884 WP_199285672.1 NZ_CP031698:3348966-3349295 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T109884 NZ_CP031698:3348966-3349295 [Xanthomonas oryzae pv. oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT109884 WP_011407754.1 NZ_CP031698:3349292-3349711 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT109884 NZ_CP031698:3349292-3349711 [Xanthomonas oryzae pv. oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA