Detailed information of TA system TA109877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3655091..3655719
• Replicon: chromosome
• Accession: NZ_CP031695
• Organism: Erwinia billingiae strain TH88

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D8MP01
• Locus tag: D0N50_RS17115
• Protein ID: WP_013201057.1
• Coordinates: 3655501..3655719 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: D8MP02
• Locus tag: D0N50_RS17110
• Protein ID: WP_013201058.1
• Coordinates: 3655091..3655471 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0N50_RS17080	3651242..3651505	+	264	WP_053141865.1	type B 50S ribosomal protein L31	-
D0N50_RS17085	3651515..3651655	+	141	WP_013201062.1	type B 50S ribosomal protein L36	-
D0N50_RS17090	3651747..3652625	-	879	WP_151038095.1	metal ABC transporter substrate-binding protein	-
D0N50_RS17095	3652622..3653488	-	867	WP_151038764.1	metal ABC transporter permease	-
D0N50_RS17100	3653491..3654177	-	687	WP_151038096.1	ABC transporter ATP-binding protein	-
D0N50_RS17105	3654593..3654946	+	354	WP_013201059.1	hypothetical protein	-
D0N50_RS17110	3655091..3655471	+	381	WP_013201058.1	Hha toxicity modulator TomB	Antitoxin
D0N50_RS17115	3655501..3655719	+	219	WP_013201057.1	hemolysin expression modulator Hha	Toxin
D0N50_RS17125	3656111..3656422	+	312	WP_151038097.1	MGMT family protein	-
D0N50_RS17130	3656447..3657004	-	558	WP_105759616.1	YbaY family lipoprotein	-
D0N50_RS17135	3657226..3658092	+	867	WP_013201054.1	acyl-CoA thioesterase II	-
D0N50_RS17140	3658181..3659470	-	1290	WP_041692221.1	ammonium transporter AmtB	-
D0N50_RS17145	3659506..3659844	-	339	WP_007886947.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.10 Da
• Isoelectric Point: 9.4825

>T109877 WP_013201057.1 NZ_CP031695:3655501-3655719 [Erwinia billingiae]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 219 bp

>T109877 NZ_CP031695:3655501-3655719 [Erwinia billingiae]
ATGACAGATAAACTTCTTACTAAAACTGATTATCTGATGCGTTTAAGACGGTGTCGGTCAATCGACACCCTCGAGCGCGT
AATCGAAAAAAACAAATACGAATTATCCGATAATGAACTGGCTGTGTTTTATTCTGCGGCAGATCACCGTCTGGCCGAAC
TGACGATGAATAAACTCTACGATAAAATCCCGGTAGCAGTTTGGAAGTTCGTTCGATAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14596.35 Da
• Isoelectric Point: 4.4699

>AT109877 WP_013201058.1 NZ_CP031695:3655091-3655471 [Erwinia billingiae]
MDEYSPKRHDIAQLKFVCENLYDESLATLGDSHHGWVNDPTAAVNLQLNDLIEHIAAFTMNYKIKHLEDSDLISQIDEYL
DDTFMLFSNYGINVQDLQRWQKSAKRLFNIFAEECALVPVQTSHSF

Nucleotide

• Length: 381 bp

>AT109877 NZ_CP031695:3655091-3655471 [Erwinia billingiae]
ATGGACGAATACTCACCAAAAAGGCATGATATCGCCCAGCTGAAATTTGTGTGTGAAAATTTGTACGATGAAAGCCTGGC
AACGTTAGGCGATAGTCATCATGGCTGGGTTAACGATCCTACTGCTGCAGTCAATTTACAGCTCAACGATCTGATTGAGC
ATATTGCTGCCTTCACCATGAATTACAAAATTAAACATCTCGAAGATAGTGACCTGATTAGTCAGATTGATGAATATCTC
GATGATACGTTTATGCTCTTTAGTAATTACGGCATTAATGTTCAGGATTTGCAGCGCTGGCAAAAATCAGCAAAACGCTT
GTTTAACATTTTCGCCGAGGAATGCGCTCTCGTGCCCGTACAGACGAGCCATTCATTTTAG

Structures

Toxin

• AlphaFold DB: D8MP01

Antitoxin

• AlphaFold DB: D8MP02