Detailed information of TA system TA109876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2976817..2977485
• Replicon: chromosome
• Accession: NZ_CP031695
• Organism: Erwinia billingiae strain TH88

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D0N50_RS13720
• Protein ID: WP_105759230.1
• Coordinates: 2976817..2976999 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D0N50_RS13725
• Protein ID: WP_151038736.1
• Coordinates: 2977072..2977485 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0N50_RS13685	2972104..2972454	-	351	WP_013201726.1	hypothetical protein	-
D0N50_RS13690	2972451..2972918	-	468	WP_013201725.1	glycoside hydrolase family protein	-
D0N50_RS13695	2973198..2973887	+	690	WP_151037754.1	helix-turn-helix transcriptional regulator	-
D0N50_RS22960	2974009..2974146	+	138	WP_191907130.1	hypothetical protein	-
D0N50_RS13700	2974155..2974442	-	288	WP_151037755.1	phage holin family protein	-
D0N50_RS13705	2974429..2974809	-	381	WP_151037756.1	phage holin family protein	-
D0N50_RS13710	2975124..2975954	+	831	WP_053145469.1	alpha/beta hydrolase	-
D0N50_RS13715	2976176..2976694	+	519	WP_151037757.1	ATP-binding protein	-
D0N50_RS13720	2976817..2976999	+	183	WP_105759230.1	type II toxin-antitoxin system HicA family toxin	Toxin
D0N50_RS13725	2977072..2977485	+	414	WP_151038736.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D0N50_RS13730	2977770..2979020	-	1251	WP_013201718.1	NADP-dependent isocitrate dehydrogenase	-
D0N50_RS13735	2979153..2979785	+	633	WP_151037758.1	23S rRNA pseudouridine(2457) synthase RluE	-
D0N50_RS13740	2979819..2980295	+	477	WP_013201716.1	NUDIX hydrolase	-
D0N50_RS13745	2980353..2981456	+	1104	WP_151037759.1	tRNA 2-thiouridine(34) synthase MnmA	-
D0N50_RS13750	2981530..2982174	+	645	WP_134823901.1	high frequency lysogenization protein HflD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2960737..2977485	16748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6734.01 Da
• Isoelectric Point: 10.7899

>T109876 WP_105759230.1 NZ_CP031695:2976817-2976999 [Erwinia billingiae]
MKSCELIRLLEKNGWILSRVKGSHHTFKHPDSCAIVTLPHPSKDIKTGTLRQILKKAGLT

Nucleotide

• Length: 183 bp

>T109876 NZ_CP031695:2976817-2976999 [Erwinia billingiae]
TTGAAAAGTTGCGAACTGATCAGGCTTTTAGAGAAAAATGGCTGGATTCTTTCCAGAGTCAAAGGCAGCCACCACACGTT
CAAGCACCCCGATTCGTGTGCCATCGTAACGCTGCCCCATCCCAGCAAAGATATTAAAACTGGTACGCTGCGGCAAATAC
TAAAAAAAGCAGGGCTTACCTGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15013.77 Da
• Isoelectric Point: 4.7826

>AT109876 WP_151038736.1 NZ_CP031695:2977072-2977485 [Erwinia billingiae]
MHYPAFIEIDKDGSASGWFPDIDGCIFAGDTIEDAIADARSAIDAHFEALAENGAELPAPHPMQEHVAANANEYVGGQWV
LVDINMDKFDGKAERINITLPHRLLHQIDNAVKSRPDYGSRSAFIAVATRKELRRAG

Nucleotide

• Length: 414 bp

>AT109876 NZ_CP031695:2977072-2977485 [Erwinia billingiae]
ATGCATTACCCAGCTTTCATCGAAATAGATAAAGACGGCTCAGCCAGTGGTTGGTTCCCCGATATTGACGGCTGCATTTT
CGCTGGCGACACAATCGAAGATGCGATTGCCGATGCCAGAAGCGCAATTGATGCCCATTTCGAGGCGCTGGCTGAAAATG
GTGCAGAACTCCCTGCTCCTCACCCAATGCAGGAACATGTCGCCGCTAATGCGAACGAATATGTCGGCGGCCAGTGGGTG
TTGGTCGACATCAACATGGATAAATTTGACGGCAAAGCCGAAAGAATAAACATTACATTGCCGCATCGGTTATTACATCA
GATTGATAATGCGGTAAAAAGTCGTCCAGATTACGGCAGCCGCAGCGCATTTATTGCAGTGGCAACACGCAAAGAACTGC
GTCGTGCCGGTTAG