Detailed information of TA system TA109873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1703961..1704551
• Replicon: chromosome
• Accession: NZ_CP031695
• Organism: Erwinia billingiae strain TH88

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: D0N50_RS07665
• Protein ID: WP_151037015.1
• Coordinates: 1704219..1704551 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D8MUA0
• Locus tag: D0N50_RS07660
• Protein ID: WP_013202892.1
• Coordinates: 1703961..1704218 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0N50_RS07635	1699303..1700739	+	1437	WP_053142154.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
D0N50_RS07640	1700768..1701997	+	1230	WP_151037011.1	MFS transporter	-
D0N50_RS07645	1702150..1703031	+	882	WP_151037012.1	siderophore-interacting protein	-
D0N50_RS07650	1703045..1703719	+	675	WP_151037013.1	DUF998 domain-containing protein	-
D0N50_RS07655	1703705..1703923	-	219	WP_191907229.1	hypothetical protein	-
D0N50_RS07660	1703961..1704218	+	258	WP_013202892.1	antitoxin	Antitoxin
D0N50_RS07665	1704219..1704551	+	333	WP_151037015.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D0N50_RS07675	1704973..1706094	+	1122	WP_013202890.1	Gfo/Idh/MocA family oxidoreductase	-
D0N50_RS07680	1706239..1706547	-	309	WP_013202889.1	helix-turn-helix domain-containing protein	-
D0N50_RS07685	1706769..1708874	+	2106	WP_151037016.1	elongation factor G	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (5-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11983.80 Da
• Isoelectric Point: 7.1684

>T109873 WP_151037015.1 NZ_CP031695:1704219-1704551 [Erwinia billingiae]
MERGEIWLVSLDPTAGHEQRGTRPVLIVSPAAFNRVTQLPVVLPVTRGGNFARTAGFIVSLDGAGTKTTGVIRCDQPRTI
DMSARHGERLERLPDDVVNEVLARLEAILI

Nucleotide

• Length: 333 bp

>T109873 NZ_CP031695:1704219-1704551 [Erwinia billingiae]
ATGGAGAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGACAGCGGGACATGAACAACGTGGGACTCGTCCGGTACTCAT
TGTATCTCCCGCCGCATTTAACCGGGTCACTCAGTTACCTGTCGTCCTTCCTGTTACCCGAGGTGGCAACTTCGCCCGCA
CAGCGGGGTTTATCGTATCACTGGACGGAGCAGGAACAAAAACAACGGGTGTGATTCGCTGCGATCAGCCGAGAACCATT
GATATGAGTGCCCGGCACGGTGAGAGACTCGAACGTCTGCCCGATGATGTTGTTAATGAAGTGCTGGCCAGACTGGAAGC
TATTCTTATTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9435.04 Da
• Isoelectric Point: 4.5982

>AT109873 WP_013202892.1 NZ_CP031695:1703961-1704218 [Erwinia billingiae]
MYITRLKKVGGSVMLAVPPAVLKTLALSTESEVGMTIKNGCLIIEPQKRPRYSLEELLAQCDPQAEMSEEDREWMDTPVA
GKEVL

Nucleotide

• Length: 258 bp

>AT109873 NZ_CP031695:1703961-1704218 [Erwinia billingiae]
ATGTATATCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTAAAAACGTTGGCGTT
GTCGACAGAAAGCGAAGTGGGCATGACCATTAAAAATGGCTGCCTGATTATTGAACCGCAAAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCACAGTGCGATCCGCAGGCTGAAATGAGCGAAGAAGATCGGGAATGGATGGATACGCCTGTGGCA
GGCAAGGAGGTCCTGTAA

Structures

Antitoxin

• AlphaFold DB: D8MUA0