Detailed information of TA system TA109864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 463466..464103
• Replicon: chromosome
• Accession: NZ_CP031694
• Organism: Bacillus velezensis strain SRCM101368

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: DXY21_RS02360
• Protein ID: WP_003156187.1
• Coordinates: 463466..463816 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: DXY21_RS02365
• Protein ID: WP_003156188.1
• Coordinates: 463822..464103 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXY21_RS02320	458506..459108	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
DXY21_RS02325	459108..459896	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
DXY21_RS02330	459862..460344	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
DXY21_RS02335	460341..460670	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
DXY21_RS02340	460734..461741	-	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
DXY21_RS02345	461753..462154	-	402	WP_003156178.1	anti-sigma regulatory factor	-
DXY21_RS02350	462157..462522	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
DXY21_RS02355	462527..463348	-	822	WP_003156182.1	STAS domain-containing protein	-
DXY21_RS02360	463466..463816	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DXY21_RS02365	463822..464103	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
DXY21_RS02370	464223..465392	-	1170	WP_160249102.1	alanine racemase	-
DXY21_RS02375	465509..466516	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
DXY21_RS02380	466681..467046	-	366	WP_003156192.1	holo-ACP synthase	-
DXY21_RS02385	467139..467738	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T109864 WP_003156187.1 NZ_CP031694:c463816-463466 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T109864 NZ_CP031694:c463816-463466 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT109864 WP_003156188.1 NZ_CP031694:c464103-463822 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT109864 NZ_CP031694:c464103-463822 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5