Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1167206..1167842 Replicon chromosome
Accession NZ_CP031693
Organism Bacillus subtilis strain SRCM101393

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag DXY22_RS05960 Protein ID WP_003156187.1
Coordinates 1167492..1167842 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag DXY22_RS05955 Protein ID WP_003225183.1
Coordinates 1167206..1167487 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXY22_RS05935 1163565..1164164 - 600 WP_129010114.1 rhomboid family intramembrane serine protease -
DXY22_RS05940 1164259..1164624 + 366 WP_014475874.1 holo-ACP synthase -
DXY22_RS05945 1164790..1165806 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
DXY22_RS05950 1165921..1167090 + 1170 WP_015252766.1 alanine racemase -
DXY22_RS05955 1167206..1167487 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
DXY22_RS05960 1167492..1167842 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DXY22_RS05965 1167957..1168781 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
DXY22_RS05970 1168786..1169151 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
DXY22_RS05975 1169155..1169556 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
DXY22_RS05980 1169568..1170575 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
DXY22_RS05985 1170637..1170966 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
DXY22_RS05990 1170963..1171445 + 483 WP_003234299.1 anti-sigma B factor RsbW -
DXY22_RS05995 1171411..1172199 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
DXY22_RS06000 1172199..1172798 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T109862 WP_003156187.1 NZ_CP031693:1167492-1167842 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T109862 NZ_CP031693:1167492-1167842 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT109862 WP_003225183.1 NZ_CP031693:1167206-1167487 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT109862 NZ_CP031693:1167206-1167487 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References