Detailed information of TA system TA109833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 728681..729318
• Replicon: chromosome
• Accession: NZ_CP031688
• Organism: Haemophilus influenzae strain P636-8296

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: -
• Locus tag: CH597_RS03745
• Protein ID: WP_070828277.1
• Coordinates: 728681..729085 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: CH597_RS03750
• Protein ID: WP_005649046.1
• Coordinates: 729082..729318 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH597_RS03715	724241..724807	+	567	WP_005544066.1	elongation factor P	-
CH597_RS03725	725158..725745	-	588	WP_005654063.1	primosomal replication protein	-
CH597_RS03730	725778..727130	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
CH597_RS03735	727444..728133	-	690	WP_011271988.1	ribonuclease T	-
CH597_RS03740	728207..728614	-	408	WP_005688980.1	lactoylglutathione lyase	-
CH597_RS03745	728681..729085	-	405	WP_070828277.1	type II toxin-antitoxin system VapC family toxin	Toxin
CH597_RS03750	729082..729318	-	237	WP_005649046.1	antitoxin	Antitoxin
CH597_RS03755	729411..730136	-	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
CH597_RS03760	730189..730707	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
CH597_RS03765	730926..732692	+	1767	WP_116962847.1	aspartate--tRNA ligase	-
CH597_RS03770	732714..733187	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
CH597_RS03775	733199..733939	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T109833 WP_070828277.1 NZ_CP031688:c729085-728681 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVIITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T109833 NZ_CP031688:c729085-728681 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTATTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT109833 WP_005649046.1 NZ_CP031688:c729318-729082 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT109833 NZ_CP031688:c729318-729082 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Antitoxin

• AlphaFold DB: E4QWH3