Detailed information of TA system TA109825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 704222..704859
• Replicon: chromosome
• Accession: NZ_CP031687
• Organism: Haemophilus influenzae strain P641-4342

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: CH602_RS03565
• Protein ID: WP_005649049.1
• Coordinates: 704222..704626 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: CH602_RS03570
• Protein ID: WP_005649046.1
• Coordinates: 704623..704859 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH602_RS03535	699780..700346	+	567	WP_005662944.1	elongation factor P	-
CH602_RS03545	700698..701285	-	588	WP_005654063.1	primosomal replication protein	-
CH602_RS03550	701318..702670	-	1353	WP_005688977.1	Na+/H+ antiporter family protein	-
CH602_RS03555	702985..703674	-	690	WP_005688979.1	ribonuclease T	-
CH602_RS03560	703748..704155	-	408	WP_005688980.1	lactoylglutathione lyase	-
CH602_RS03565	704222..704626	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
CH602_RS03570	704623..704859	-	237	WP_005649046.1	antitoxin	Antitoxin
CH602_RS03575	704952..705677	-	726	WP_005688983.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
CH602_RS03580	705730..706248	-	519	WP_005688985.1	isoprenylcysteine carboxyl methyltransferase family protein	-
CH602_RS03585	706468..708234	+	1767	WP_005688987.1	aspartate--tRNA ligase	-
CH602_RS03590	708296..709168	+	873	WP_005688989.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T109825 WP_005649049.1 NZ_CP031687:c704626-704222 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T109825 NZ_CP031687:c704626-704222 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT109825 WP_005649046.1 NZ_CP031687:c704859-704623 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT109825 NZ_CP031687:c704859-704623 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3