Detailed information of TA system TA109754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 242927..243432
• Replicon: chromosome
• Accession: NZ_CP031677
• Organism: Pseudomonas aeruginosa strain E80

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: DOM02_RS01170
• Protein ID: WP_003083773.1
• Coordinates: 242927..243208 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: DOM02_RS01175
• Protein ID: WP_003083775.1
• Coordinates: 243205..243432 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOM02_RS01145	238178..239527	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
DOM02_RS01150	239576..240262	+	687	WP_003083762.1	FadR family transcriptional regulator	-
DOM02_RS01155	240363..241097	+	735	WP_023081667.1	GntR family transcriptional regulator	-
DOM02_RS01160	241301..241687	+	387	WP_014602345.1	aegerolysin family protein	-
DOM02_RS01165	241719..242627	-	909	WP_003083769.1	LysR family transcriptional regulator	-
DOM02_RS01170	242927..243208	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
DOM02_RS01175	243205..243432	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
DOM02_RS01180	243608..244228	-	621	WP_003101226.1	hypothetical protein	-
DOM02_RS01185	244329..244829	+	501	WP_003101228.1	LEA type 2 family protein	-
DOM02_RS01190	244902..245243	+	342	WP_023081666.1	alkylphosphonate utilization protein	-
DOM02_RS01195	245343..245927	-	585	WP_073674849.1	DUF4276 family protein	-
DOM02_RS01200	245924..247132	-	1209	WP_023102401.1	AAA family ATPase	-
DOM02_RS01205	247524..247637	+	114	Protein_234	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	247524..247640	116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T109754 WP_003083773.1 NZ_CP031677:c243208-242927 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T109754 NZ_CP031677:c243208-242927 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATTGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT109754 WP_003083775.1 NZ_CP031677:c243432-243205 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT109754 NZ_CP031677:c243432-243205 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9