Detailed information of TA system TA109749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 971209..972000
• Replicon: chromosome
• Accession: NZ_CP031673
• Organism: Staphylococcus aureus strain MOZ66

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: DD548_RS05150
• Protein ID: WP_000525003.1
• Coordinates: 971209..971673 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0E7YIA0
• Locus tag: DD548_RS05155
• Protein ID: WP_000333630.1
• Coordinates: 971686..972000 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD548_RS05130	967260..967790	+	531	WP_000184383.1	acyl-CoA thioesterase	-
DD548_RS05135	968050..969195	-	1146	WP_001245441.1	radical SAM/CxCxxxxC motif protein YfkAB	-
DD548_RS05140	969240..970286	-	1047	WP_001145722.1	site-specific integrase	-
DD548_RS05145	970497..971177	-	681	WP_000392109.1	type II toxin-antitoxin system PemK/MazF family toxin	-
DD548_RS05150	971209..971673	-	465	WP_000525003.1	hypothetical protein	Toxin
DD548_RS05155	971686..972000	-	315	WP_000333630.1	helix-turn-helix domain-containing protein	Antitoxin
DD548_RS05160	972153..972389	+	237	WP_001121116.1	helix-turn-helix domain-containing protein	-
DD548_RS05165	972403..972582	+	180	WP_000438352.1	hypothetical protein	-
DD548_RS05170	972683..973165	-	483	WP_000394410.1	hypothetical protein	-
DD548_RS05175	973225..973968	+	744	WP_001148594.1	phage antirepressor KilAC domain-containing protein	-
DD548_RS05180	974144..974374	-	231	WP_000395455.1	hypothetical protein	-
DD548_RS05185	974445..974666	+	222	WP_000594789.1	hypothetical protein	-
DD548_RS05190	974659..974820	+	162	WP_000066011.1	DUF1270 domain-containing protein	-
DD548_RS05195	974917..975177	+	261	WP_000291090.1	DUF1108 family protein	-
DD548_RS05200	975187..975408	+	222	WP_000815403.1	DUF2483 family protein	-
DD548_RS05205	975401..976189	+	789	WP_088591918.1	ATP-binding protein	-
DD548_RS05210	976218..976769	+	552	WP_000704705.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	967260..1011466	44206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 18156.52 Da
• Isoelectric Point: 4.7927

>T109749 WP_000525003.1 NZ_CP031673:c971673-971209 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKLHHID

Nucleotide

• Length: 465 bp

>T109749 NZ_CP031673:c971673-971209 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAGGCAGGGATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCTGTTCCATTACGCGAAATTGTAGAAGCTTATAATTATGGCGTACGTAACTTGTATGAATT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCGATCACATTTGAGCCGTTGAGAGTTTTTAAATTGCATCACATTGATTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.56 Da
• Isoelectric Point: 6.4736

>AT109749 WP_000333630.1 NZ_CP031673:c972000-971686 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 315 bp

>AT109749 NZ_CP031673:c972000-971686 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCATCTT
GAAGGAGAATTGACAGATGACGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E7YIA0