Detailed information of TA system TA109745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 780608..781137
• Replicon: chromosome
• Accession: NZ_CP031673
• Organism: Staphylococcus aureus strain MOZ66

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DD548_RS03970
• Protein ID: WP_000621175.1
• Coordinates: 780775..781137 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: DD548_RS03965
• Protein ID: WP_000948331.1
• Coordinates: 780608..780778 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD548_RS03935	775644..776204	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
DD548_RS03940	776413..776892	+	480	WP_001287088.1	hypothetical protein	-
DD548_RS03945	776885..778468	+	1584	WP_117224535.1	PH domain-containing protein	-
DD548_RS03950	778455..778946	+	492	WP_001205910.1	PH domain-containing protein	-
DD548_RS03955	778950..779309	+	360	WP_000581200.1	holo-ACP synthase	-
DD548_RS03960	779375..780523	+	1149	WP_001281145.1	alanine racemase	-
DD548_RS03965	780608..780778	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
DD548_RS03970	780775..781137	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DD548_RS03980	781486..782487	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
DD548_RS03985	782606..782932	+	327	WP_001052491.1	anti-sigma factor antagonist	-
DD548_RS03990	782934..783413	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
DD548_RS03995	783388..784158	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T109745 WP_000621175.1 NZ_CP031673:780775-781137 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T109745 NZ_CP031673:780775-781137 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT109745 WP_000948331.1 NZ_CP031673:780608-780778 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT109745 NZ_CP031673:780608-780778 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1