Detailed information of TA system TA109649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 152660..153165
• Replicon: chromosome
• Accession: NZ_CP031659
• Organism: Pseudomonas aeruginosa strain PABL012

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: DZ894_RS00705
• Protein ID: WP_003083773.1
• Coordinates: 152660..152941 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: DZ894_RS00710
• Protein ID: WP_003083775.1
• Coordinates: 152938..153165 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZ894_RS00680	147911..149260	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
DZ894_RS00685	149309..149995	+	687	WP_003083762.1	FadR family transcriptional regulator	-
DZ894_RS00690	150096..150830	+	735	WP_003101224.1	GntR family transcriptional regulator	-
DZ894_RS00695	151034..151420	+	387	WP_014602345.1	aegerolysin family protein	-
DZ894_RS00700	151452..152360	-	909	WP_003083769.1	LysR family transcriptional regulator	-
DZ894_RS00705	152660..152941	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
DZ894_RS00710	152938..153165	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
DZ894_RS00715	153341..153961	-	621	WP_003101226.1	hypothetical protein	-
DZ894_RS00720	154062..154562	+	501	WP_003101228.1	LEA type 2 family protein	-
DZ894_RS00725	154635..154976	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
DZ894_RS00730	155058..156485	-	1428	WP_003083784.1	GABA permease	-
DZ894_RS00735	156654..158147	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T109649 WP_003083773.1 NZ_CP031659:c152941-152660 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T109649 NZ_CP031659:c152941-152660 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT109649 WP_003083775.1 NZ_CP031659:c153165-152938 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT109649 NZ_CP031659:c153165-152938 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9