Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 61916..62517 Replicon plasmid pCARB35_01
Accession NZ_CP031654
Organism Escherichia coli strain UK_Dog_Liverpool

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag DL545_RS25535 Protein ID WP_001216045.1
Coordinates 61916..62296 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DL545_RS25540 Protein ID WP_001190712.1
Coordinates 62296..62517 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL545_RS25510 57357..58841 - 1485 WP_000124150.1 terminase -
DL545_RS25515 58841..60034 - 1194 WP_000219625.1 hypothetical protein -
DL545_RS25520 60120..60572 - 453 WP_001326849.1 late promoter-activating protein -
DL545_RS25525 60661..61704 - 1044 WP_023356283.1 DUF968 domain-containing protein -
DL545_RS25530 61732..61911 - 180 WP_001339207.1 hypothetical protein -
DL545_RS25535 61916..62296 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DL545_RS25540 62296..62517 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DL545_RS25545 62590..62979 - 390 WP_000506730.1 DNA repair protein -
DL545_RS25550 63154..63369 + 216 Protein_59 DNA-binding protein -
DL545_RS25555 63371..63745 + 375 Protein_60 integrase core domain-containing protein -
DL545_RS25560 64316..66172 - 1857 WP_077902027.1 acyltransferase -
DL545_RS25565 66398..67144 + 747 Protein_62 IS1 family transposase -
DL545_RS27265 67243..67422 - 180 Protein_63 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..89643 89643
- flank IS/Tn - - 63371..63745 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T109644 WP_001216045.1 NZ_CP031654:c62296-61916 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T109644 NZ_CP031654:c62296-61916 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT109644 WP_001190712.1 NZ_CP031654:c62517-62296 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT109644 NZ_CP031654:c62517-62296 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References