Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 972963..973617 Replicon chromosome
Accession NZ_CP031653
Organism Escherichia coli strain UK_Dog_Liverpool

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag DL545_RS05300 Protein ID WP_000244777.1
Coordinates 973210..973617 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DL545_RS05295 Protein ID WP_000354046.1
Coordinates 972963..973229 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL545_RS05270 968251..968994 + 744 Protein_950 SDR family oxidoreductase -
DL545_RS05275 969051..970484 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
DL545_RS05280 970529..970840 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
DL545_RS05285 971004..971663 + 660 WP_000250274.1 hemolysin III family protein -
DL545_RS05290 971740..972720 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
DL545_RS05295 972963..973229 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DL545_RS05300 973210..973617 + 408 WP_000244777.1 toxin CptA Toxin
DL545_RS05305 973657..974178 - 522 WP_001055874.1 flavodoxin FldB -
DL545_RS05310 974290..975186 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DL545_RS05315 975211..975921 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DL545_RS05320 975927..977660 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T109627 WP_000244777.1 NZ_CP031653:973210-973617 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T109627 NZ_CP031653:973210-973617 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT109627 WP_000354046.1 NZ_CP031653:972963-973229 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT109627 NZ_CP031653:972963-973229 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References