Detailed information of TA system TA109624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 413017..413817
• Replicon: chromosome
• Accession: NZ_CP031653
• Organism: Escherichia coli strain UK_Dog_Liverpool

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: DL545_RS02260
• Protein ID: WP_000342449.1
• Coordinates: 413290..413817 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: DL545_RS02255
• Protein ID: WP_116995140.1
• Coordinates: 413017..413283 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL545_RS02235	408674..409342	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
DL545_RS02240	409335..410393	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
DL545_RS02245	410638..411492	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
DL545_RS02250	411764..412867	+	1104	WP_001350438.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
DL545_RS02255	413017..413283	+	267	WP_116995140.1	DUF1778 domain-containing protein	Antitoxin
DL545_RS02260	413290..413817	+	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
DL545_RS02265	413814..414197	-	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
DL545_RS02270	414621..415730	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
DL545_RS02275	415778..416704	+	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
DL545_RS02280	416701..417978	+	1278	WP_000803799.1	branched chain amino acid ABC transporter permease LivM	-
DL545_RS02285	417975..418742	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T109624 WP_000342449.1 NZ_CP031653:413290-413817 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T109624 NZ_CP031653:413290-413817 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.30 Da
• Isoelectric Point: 8.7683

>AT109624 WP_116995140.1 NZ_CP031653:413017-413283 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEEIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT109624 NZ_CP031653:413017-413283 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGAGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4