Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 243524..244049 Replicon chromosome
Accession NZ_CP031653
Organism Escherichia coli strain UK_Dog_Liverpool

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DL545_RS01385 Protein ID WP_001159871.1
Coordinates 243744..244049 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag DL545_RS01380 Protein ID WP_000813630.1
Coordinates 243524..243742 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL545_RS01345 238723..239420 + 698 Protein_240 IS1-like element IS1A family transposase -
DL545_RS01355 239674..240696 - 1023 WP_000361402.1 helicase UvrD -
DL545_RS01360 240681..242246 - 1566 WP_001128474.1 AAA family ATPase -
DL545_RS01365 242321..242737 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
DL545_RS01370 242734..242964 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
DL545_RS01380 243524..243742 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DL545_RS01385 243744..244049 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DL545_RS01390 244050..244856 + 807 WP_000016970.1 site-specific integrase -
DL545_RS01395 245578..246333 + 756 WP_000852146.1 replication initiation protein RepE -
DL545_RS01400 246921..248087 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 239674..263065 23391


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T109623 WP_001159871.1 NZ_CP031653:243744-244049 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T109623 NZ_CP031653:243744-244049 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT109623 WP_000813630.1 NZ_CP031653:243524-243742 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT109623 NZ_CP031653:243524-243742 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References