Detailed information of TA system TA109615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 78914..79467
• Replicon: plasmid unnamed3
• Accession: NZ_CP031652
• Organism: Pantoea agglomerans strain TH81

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: D0A61_RS23040
• Protein ID: WP_122460053.1
• Coordinates: 78914..79228 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: D0A61_RS23045
• Protein ID: WP_122460054.1
• Coordinates: 79231..79467 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0A61_RS23000	73983..74596	-	614	Protein_60	LysE family translocator	-
D0A61_RS23005	74694..75503	-	810	WP_122460051.1	AraC family transcriptional regulator	-
D0A61_RS23010	75508..75804	-	297	WP_122460052.1	chorismate mutase	-
D0A61_RS23015	76059..76400	-	342	Protein_63	DsbA family protein	-
D0A61_RS23020	76398..76781	+	384	Protein_64	hypothetical protein	-
D0A61_RS23025	76781..76996	+	216	Protein_65	hypothetical protein	-
D0A61_RS23035	77960..78729	-	770	Protein_66	thioredoxin domain-containing protein	-
D0A61_RS23040	78914..79228	-	315	WP_122460053.1	CcdB family protein	Toxin
D0A61_RS23045	79231..79467	-	237	WP_122460054.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
D0A61_RS23050	79611..80120	-	510	WP_122460055.1	hypothetical protein	-
D0A61_RS23055	80152..80397	-	246	WP_122460056.1	hypothetical protein	-
D0A61_RS23060	80490..80789	-	300	WP_122460057.1	hypothetical protein	-
D0A61_RS23065	81450..82173	-	724	Protein_72	complement resistance protein TraT	-
D0A61_RS23070	82265..82873	-	609	WP_122460058.1	hypothetical protein	-
D0A61_RS23075	82960..83764	-	805	Protein_74	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..152523	152523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-102)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11680.64 Da
• Isoelectric Point: 6.4541

>T109615 WP_122460053.1 NZ_CP031652:c79228-78914 [Pantoea agglomerans]
MQFTVYSNTGKSTVYPLLLDVTSDIIVQLNHRIVIPLLPVEKYPSSTRPERLVPLIRLTDDKEYAVMTHEMASIPARALG
AVFCNASLYRTEIKAAIDFLLDSI

Nucleotide

• Length: 315 bp

>T109615 NZ_CP031652:c79228-78914 [Pantoea agglomerans]
ATGCAGTTCACTGTATACAGTAATACCGGGAAAAGTACCGTCTATCCTCTACTGCTCGATGTTACCAGCGATATTATCGT
GCAGTTAAACCACAGGATAGTGATCCCCCTGCTACCTGTTGAAAAGTACCCGAGCAGCACGCGCCCTGAACGGCTGGTTC
CGCTTATCAGACTGACTGATGATAAAGAATATGCCGTAATGACGCATGAAATGGCGAGCATTCCCGCTCGTGCTTTGGGG
GCTGTGTTCTGCAATGCTTCTCTCTATCGTACAGAGATTAAAGCTGCTATCGATTTTCTTCTCGACAGCATCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9021.04 Da
• Isoelectric Point: 4.6234

>AT109615 WP_122460054.1 NZ_CP031652:c79467-79231 [Pantoea agglomerans]
MVITQRATQSVTVTVERDLLSRAREAGINLSATLTMALDAELRQYEVKKWQEENSEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT109615 NZ_CP031652:c79467-79231 [Pantoea agglomerans]
ATGGTTATAACACAACGGGCAACACAAAGCGTTACCGTGACCGTAGAACGTGACTTACTCAGCCGTGCGCGTGAGGCCGG
AATTAATCTCAGCGCCACCCTGACCATGGCCCTTGACGCTGAACTTCGTCAGTATGAAGTGAAAAAATGGCAGGAGGAAA
ACAGCGAAGCGCTGGAAGCTCTAAACCGCTTTCACGATGAACACGGTTGTTTCAGCGATGAGTACAGGACATTTTAA