Detailed information of TA system TA109609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3414731..3415362
• Replicon: chromosome
• Accession: NZ_CP031649
• Organism: Pantoea agglomerans strain TH81

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7X5MR53
• Locus tag: D0A61_RS16210
• Protein ID: WP_010253953.1
• Coordinates: 3415183..3415362 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8X8DTB6
• Locus tag: D0A61_RS16205
• Protein ID: WP_010253955.1
• Coordinates: 3414731..3415156 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0A61_RS16185	3411434..3411943	-	510	WP_039391324.1	phenolic acid decarboxylase	-
D0A61_RS16190	3412049..3412948	+	900	WP_122459250.1	LysR family transcriptional regulator	-
D0A61_RS16195	3413394..3414323	-	930	WP_045140772.1	Rpn family recombination-promoting nuclease/putative transposase	-
D0A61_RS16200	3414509..3414649	-	141	WP_033756575.1	CRISPR-associated protein Cas1	-
D0A61_RS16205	3414731..3415156	-	426	WP_010253955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D0A61_RS16210	3415183..3415362	-	180	WP_010253953.1	type II toxin-antitoxin system HicA family toxin	Toxin
D0A61_RS16215	3415545..3416969	-	1425	WP_010253950.1	dihydrolipoyl dehydrogenase	-
D0A61_RS16220	3417130..3419031	-	1902	WP_122459252.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6467.52 Da
• Isoelectric Point: 10.7891

>T109609 WP_010253953.1 NZ_CP031649:c3415362-3415183 [Pantoea agglomerans]
MDSSTLISEMKADGWVLTGINGSHHHFTHPTKPGLVTVPHPKKDLPIGTVKSIRKQARI

Nucleotide

• Length: 180 bp

>T109609 NZ_CP031649:c3415362-3415183 [Pantoea agglomerans]
ATGGATAGCAGCACGTTAATTTCCGAAATGAAGGCTGATGGCTGGGTTCTGACAGGGATAAACGGTAGCCATCACCATTT
CACACATCCGACAAAACCGGGGCTGGTTACAGTACCCCATCCTAAAAAAGACTTACCGATCGGTACGGTAAAGAGCATCA
GGAAACAAGCGAGAATTTAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15422.60 Da
• Isoelectric Point: 4.7662

>AT109609 WP_010253955.1 NZ_CP031649:c3415156-3414731 [Pantoea agglomerans]
MFYPIAIEAGDHEHAYGVIVPDLPGCFSAGDTLDEAIKNAKEAITGHIELCVELGHEIPAVSTIETLATNPDYQGYIWAL
VDVDVIRLLGGSEKINVTLPRSLIDRIDRCVASHPEYKSRSGFLAQVALERITAPINRPQK

Nucleotide

• Length: 426 bp

>AT109609 NZ_CP031649:c3415156-3414731 [Pantoea agglomerans]
ATGTTTTATCCAATCGCGATTGAAGCGGGTGATCACGAACACGCATACGGCGTAATCGTTCCCGATTTACCAGGGTGTTT
CTCTGCCGGGGATACTCTTGATGAAGCCATTAAAAACGCCAAAGAGGCTATCACAGGGCATATCGAATTATGTGTAGAAC
TCGGTCATGAGATCCCTGCCGTGTCCACCATTGAGACTCTGGCCACGAATCCTGACTATCAGGGTTATATCTGGGCATTG
GTTGATGTAGATGTCATTCGCTTGCTGGGCGGCTCTGAAAAAATCAATGTCACTTTACCCAGGTCGTTAATTGATCGGAT
TGATCGTTGTGTGGCTTCACACCCCGAATACAAAAGTCGCTCGGGCTTTCTGGCTCAGGTAGCGCTTGAGCGTATTACTG
CTCCGATTAACAGGCCTCAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7X5MR53