Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3158905..3159522 Replicon chromosome
Accession NZ_CP031649
Organism Pantoea agglomerans strain TH81

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag D0A61_RS14970 Protein ID WP_003850458.1
Coordinates 3159307..3159522 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag D0A61_RS14965 Protein ID WP_003850455.1
Coordinates 3158905..3159282 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0A61_RS14935 3155212..3155466 + 255 WP_010671219.1 type B 50S ribosomal protein L31 -
D0A61_RS14940 3155478..3155618 + 141 WP_010255896.1 type B 50S ribosomal protein L36 -
D0A61_RS14945 3155664..3156542 - 879 WP_033785427.1 metal ABC transporter substrate-binding protein -
D0A61_RS14950 3156558..3157397 - 840 WP_010255890.1 metal ABC transporter permease -
D0A61_RS14955 3157394..3158062 - 669 WP_033766689.1 ABC transporter ATP-binding protein -
D0A61_RS14960 3158404..3158757 + 354 WP_122459125.1 hypothetical protein -
D0A61_RS14965 3158905..3159282 + 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
D0A61_RS14970 3159307..3159522 + 216 WP_003850458.1 hemolysin expression modulator Hha Toxin
D0A61_RS14980 3159929..3160240 + 312 WP_031293095.1 MGMT family protein -
D0A61_RS14985 3160280..3160837 - 558 WP_010255881.1 YbaY family lipoprotein -
D0A61_RS14990 3161028..3161891 + 864 WP_010255880.1 acyl-CoA thioesterase II -
D0A61_RS14995 3161942..3163228 - 1287 WP_010255877.1 ammonium transporter AmtB -
D0A61_RS15000 3163263..3163601 - 339 WP_003850469.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T109608 WP_003850458.1 NZ_CP031649:3159307-3159522 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T109608 NZ_CP031649:3159307-3159522 [Pantoea agglomerans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGCTGCCGCTCTCTTGATACCCTGGAAAGGGTGAT
TGAAAAAAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTTTACTCAGCAGCTGACCATCGCCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCGGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT109608 WP_003850455.1 NZ_CP031649:3158905-3159282 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT109608 NZ_CP031649:3158905-3159282 [Pantoea agglomerans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTTTGTGAGAGTCTGTTTGACGACAGCATGGC
AACATTAACCGACAGTCATCATGGCTGGGTTAATGATCCCACTTCTGAAAGCAATCTACAGCTTAACGATTTGATTGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAATTATGGTGTCAATAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References