Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 134247..134983 Replicon plasmid pZYST1C1
Accession NZ_CP031614
Organism Klebsiella pneumoniae strain ZYST1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag DWV03_RS27135 Protein ID WP_003026803.1
Coordinates 134247..134729 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DWV03_RS27140 Protein ID WP_003026799.1
Coordinates 134717..134983 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWV03_RS27115 129957..130361 - 405 WP_064145647.1 helix-turn-helix domain-containing protein -
DWV03_RS27120 130410..131756 - 1347 WP_077267029.1 ISNCY family transposase -
DWV03_RS27125 131982..132614 + 633 WP_001567369.1 hypothetical protein -
DWV03_RS27130 132643..134046 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
DWV03_RS27135 134247..134729 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
DWV03_RS27140 134717..134983 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DWV03_RS27145 135228..135662 - 435 WP_001567367.1 cell envelope integrity protein TolA -
DWV03_RS27150 135709..136257 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
DWV03_RS27160 136751..137059 + 309 WP_001568014.1 hypothetical protein -
DWV03_RS27170 137292..137786 - 495 WP_001568016.1 hypothetical protein -
DWV03_RS27175 137817..138389 - 573 WP_001568017.1 hypothetical protein -
DWV03_RS27180 138386..138634 - 249 WP_001568018.1 hypothetical protein -
DWV03_RS27185 138992..139342 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
DWV03_RS27190 139392..139754 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 - 1..196414 196414


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T109563 WP_003026803.1 NZ_CP031614:c134729-134247 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T109563 NZ_CP031614:c134729-134247 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT109563 WP_003026799.1 NZ_CP031614:c134983-134717 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT109563 NZ_CP031614:c134983-134717 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References