Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 684419..685098 Replicon chromosome
Accession NZ_CP031613
Organism Klebsiella pneumoniae strain ZYST1

Toxin (Protein)


Gene name ykfI Uniprot ID A0A0C7K7A4
Locus tag DWV03_RS03475 Protein ID WP_020324801.1
Coordinates 684757..685098 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0C7KEL2
Locus tag DWV03_RS03470 Protein ID WP_020324792.1
Coordinates 684419..684736 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWV03_RS03445 679633..682785 + 3153 WP_022631414.1 AIDA repeat-containing protein -
DWV03_RS03450 682878..683117 + 240 WP_020324820.1 DUF905 domain-containing protein -
DWV03_RS03455 683220..683678 + 459 WP_020324813.1 antirestriction protein -
DWV03_RS03460 683694..684170 + 477 WP_020324797.1 RadC family protein -
DWV03_RS03465 684179..684406 + 228 WP_020324796.1 DUF987 domain-containing protein -
DWV03_RS03470 684419..684736 + 318 WP_020324792.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
DWV03_RS03475 684757..685098 + 342 WP_020324801.1 TA system toxin CbtA family protein Toxin
DWV03_RS03480 685214..686047 + 834 WP_020324805.1 DUF4942 domain-containing protein -
DWV03_RS03490 686350..686856 + 507 WP_002917636.1 G/U mismatch-specific DNA glycosylase -
DWV03_RS03495 686956..688797 - 1842 WP_004174339.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 652963..686047 33084


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-103)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12818.91 Da        Isoelectric Point: 9.1484

>T109547 WP_020324801.1 NZ_CP031613:684757-685098 [Klebsiella pneumoniae]
MKTLPAIISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDETVIQEHIDAGITLADAVNFLVEKYGLVRIDRNGF
VWQEQSPYLRAVDILRARQATGLLRQSRNNAIR

Download         Length: 342 bp

>T109547 NZ_CP031613:684757-685098 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11915.51 Da        Isoelectric Point: 6.6246

>AT109547 WP_020324792.1 NZ_CP031613:684419-684736 [Klebsiella pneumoniae]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASMTGKFSDAECRKLDETFPHFISQMESMLITGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPIQR

Download         Length: 318 bp

>AT109547 NZ_CP031613:684419-684736 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7K7A4

Antitoxin

Source ID Structure
AlphaFold DB A0A0C7KEL2

References